花椒愈伤组织诱导及细胞悬浮培养研究.pdfVIP

西北林学院学报 2016 ， 31(4): 153~156 Journal of Northwest Forestry University doi:10. 3969/j. issn.1001-746 1. 2016.04.26 花椒愈伤组织诱导及细胞悬浮培养研究 周正君，田 晶，李周岐提 (西北农林科技大学西部环境与生态教育部重点实验室，陕西杨陵 712100) 摘 要:以花椒试管苗叶片为试验材料，研究了不同激素浓度对花椒愈伤组织继代培养的影响，并 以多次继代得到的疏松、活性高的愈伤组织建立花椒细胞悬浮系。结果表明:花椒愈伤组织最适继 代培养基为 MS十2 ， 4-D 1. 0 mg. L-] 十6-BA O. 5 mg • L一 1 十条糖 30 g • L-] 十琼脂 6 g • L -] ;花 椒细胞悬浮培养的最佳培养基和培养条件为 :MS十2 ,4-D 1. 0 mg • L一] + 6-BA 1. 0 mg • L一]+葡 萄糖 30 g L一 1 ，接种量 0.05 g • mL -] ，摇床转这 110 r min-] , 25 C 暗培养，该条件下悬浮细胞 生长良好，最大生长量为 4.52 g; 花椒悬浮细胞生长呈S型曲线，最适继代周期为 16 d o 关键词:花椒;愈伤组织;继代培养;细胞悬浮培养 文章编号:1001-7461(2016)04-0153-04 文献标志码:A :S573.9 中图分类号 Studies on the Callus lnduction and Cell Suspension Culture of Zanthoxylum bungeanum ZHOU Zheng-jun ,TIAN Jing ,LI Zhou-qi赘 (Key Laboratory of Environment and Ecology in Western China .Ministry of Educatzon •Northwest A F University. Yangling .Shaanxi 712100 .China) Abstract: Plantlet leaves of Zanthoxylum bungeanum were taken as experimental materials for studying the effects of different concentrations of hormone on callus induction and subculture. The crisp callus was se- lected to establish cell suspension culture. The results showed that the best callus subculture medium was MS+2 ,4-D 1. 0 mg • L -] +6-BA 0.5 mg • L-] 十sucrose 30 g • L -] + agar 6 g • L -]. The best suspension culture medium for Z. bungeanum cells was MS+2 ,4-D 1. 0 mg • L -] +6-BA 1. 0 mg • L-] 十glucose 30 g • L一] • The best culturing conditions were: inoculum size: O. 05 g • mL-1 ,gyratory shaker rotation speed: 110 r min一 1 ， temperature: 25 oC