酶的提取及其催化活性研究.pdfVIP

Agricultural Science Technology, 2016 , 17(12): 2893 2895 Copyright ( 2016 , Information Institute 01 HAAS. AII rights reserved Molecular Biology and Tissue C ulture Study on Extraction and Catalytic Activity of Tyrosinase Ningfang WANG . Qinghai Normal University, Xining 810008, China Abstract [Objective] Tyrosinase is a kind 01 polyphenol oxidase us创 widely， and 酶的提取及其催化活性研究 the study 01 tyrosinase attracts extensive attenti∞ (Method) In this expelÌment, A conversion kinetic curve 01 dopa soluti∞ was obtained using absorbance 01 potato 王宁芳. (青海师范大学，青海西宁 81仪附8) extract at 480 nm in Na:.HP0 -HCI buffer system at a pH value 01 7.2 with the valÌ- 摘 要 [目的1M氨酸酶是一种多l盼氧化酶， 4 ation rate 01 absorbance against time (ð.AIð.t) as reaction rate, and the activity 01 具有广泛用途，因此对酶氨酸酶的研究受到广 tyrosinase could thus be 臼Iculated. [Result] The results sh侧ed that at the wave- 泛重视。 [方法]试验以 pH 7.2 的 Nl地HPO.-HCI length, in the Na:.HP0 -HCI bufler system with a pH value at 7.2, there was little in- 4 缓冲溶液为体系，用分光光度计在4-80nm 处J1J terterence to the system , the data showed small fluctuations , and the activity 01 ty- rosinase was high and stable, lu时her providing a theoret阳I 1αmdation lor the de- 定土豆提取液的吸光度，以收光度对时间的变 termination and study 01 lactors inlluencing tyrosinase activity. [臼nclusion] The 化率(ð.Alår) 为反应速率，建立多巴溶液的转 catalyt比 activity 01 tyrosinase was studied and determined with buffer solution as 换动力学曲线，从而可计算出黯氨酸酶的活 substrate with high a∞uracy and good effect 性。 [结果]结果显示在此波长下，以 pH 7.2 的 Key words Absorbance ; Kinet