青稞酒中甜蜜素检测方法的研究.pdfVIP

5656 酿酒科技 2016 年第12期（总第270 期）·LIQUOR MAKING SCIENCE TECHNOLOGY 2016 No.12(Tol.270) DOI：10.13746/j.njkj.2016274 青稞酒中甜蜜素检测方法的研究 蔡林森 (青海省产品质量监督检验所，青海西宁810001) 摘 要： 采用气相色谱法测定青稞酒中甜蜜素的含量，通过优化衍生化条件，建立了青稞酒中甜蜜素准确定量的 检测方法。甜蜜素在酸性条件下和亚硝酸钠发生反应，生成易气化的环己醇亚硝酸酯。若衍生化条件控制不当， 衍生产物将产生2 个色谱峰，导致定量不准确。本实验通过优化甜蜜素的提取条件和衍生化条件，建立了一种准 确检测青稞酒中甜蜜素的方法，同时用青稞酒配制甜蜜素标准溶液，有效减小了基质效应对实验结果的影响。将 不同浓度的甜蜜素加入青稞酒中进行回收率和精密度分析，回收率范围为92.5 %～105.0 %，RSD 为1.50 %。 关键词：甜蜜素； 青稞酒； 气相色谱法； 检测 中图分类号：TS262.3 ；TS261.7 ；TS261.4 文献标识码：A 文章编号：1001-9286 （2016 ）12-0056-03 DeterminationofSodiumCyclamateinHighlandBarleyLiquor CAI Linsen (Qinghai Product Quality Supervision and Inspection Institute, Xining, Qinghai 810001,China) Abstract: The content of sodium cyclamate in highland barley liquor was determined by gas chromatography. Sodium cyclamate reacted with sodium nitrite under acid conditions and further formed easily gasified cyclohexanol nitrite. Inappropriate control of derivatization conditions may produce two chromatographic peaks of derivatives, which would result in inaccurate quantitation. In the experiment, through the optimiza tion of the extracting conditions and the derivatization of sodium cyclamate, a method for accurate determination of sodium cyclamate in high land barley liquor had been developed. Meanwhile, the preparation of cyclamate standard solution could effectively reduce matrix effects. Sodi um cyclamate of different concentration was added in highland barley liquor for recovery rate and RSD analysis, and the recovery rate was 92.5 % to 105.0 %, and RSD was 1.50 %. Keywords: sodium cyclamate; highland barley liquor; gas chromatography; detection 环己基氨基磺酸钠（俗名甜蜜素 Sodium Cyclamate） 公司；氯化钠/亚硝酸钠，天津市永大化学试剂有限公司； 是 种常用的甜味剂，属于 营