响应面法优化酶辅助提取废弃烟叶中烟碱.pdf

山东农业大学学报(自然科学版),2017,48(2):203-209 VOL.48NO.22017 Journal of ShandongAgriculturalUniversity (Natural ScienceEdition ) doi:10.3969/j.issn.1000-2324.2017.02.008 响应面法优化酶辅助提取废弃烟叶中烟碱 * 吴映捷 吕新美闫兵兵胡 鲲杨先乐, , , , 上海海洋大学 水产与生命学院, 国家水生动物病原库, 上海 201306 摘 要: 为了从废弃烟叶高效提取烟碱，本文利用响应面法对在废弃烟叶中烟碱的提取条件进行优化。以烟碱的得率 pH 4 3 Design Expert 8.0.6 为评价指标，设计酶解时间、酶解温度， 值及提取时间 因素 水平的实验模型，采用 分析 软件对烟碱的提取条件进行优化研究。优选出最佳提取工艺为：酶解时间2.0h，酶解温度48.50℃，酶用量2.10%， pH4.00。测定烟碱的得率为3.320%，与预测值3.32836%偏差较小，为废弃烟叶的烟碱提取提供理论依据。 关键词: 烟碱; 废弃烟叶; 响应面法 中图法分类号:S572 文献标识码:A 文章编号:1000-2324(2017)02-0203-07 Optimization of Nicotine Extracted from Abandoned Tobacco withResponse SurfaceMethod WUYing-jie,LVXin-mei,YAN Bing-bing,HU Kun,YANGXian-le* Collegeof FisheriesandLifeScience/ShanghaiOcean University,NationalAquaticAnimalPathogens,Shanghai201306,China Abstract:To optimize the conditions for purification ofNicotine in abandonedTobacco.Response surface method wasused to design the test models with four factors including enzyme time, enzyme temperature, enzyme dosage, ultrasonic time at three levels based on the single factor experiment and the yield of Nicotine was used as the evaluation index to optimize purification process of Nicotine. The optimum extraction condition were as follows: enzymolysis time was 2.00 h, enzymolysis temperature was 48.50 ℃, cellulose enzyme amount was 2.10%, pH was 4.00.Under this condition, the yield was up to 3.320%, the relative error was smaller compared to the predicted value 3.32836%. The optimum extraction conditionswere simpleand stableandtheywere servedasareference for industriallarge scaleproduc