海带加工副产物褐藻胶的综合利用.pdfVIP

Abstract Abstract Alginatehasbeenwidelyusedasanaturalmacromoleculematerial.Itistheuniquebiological polysaccharide in brown algae.In this article, Sodium alginate was extracted by using alkali extraction from the by-productsof Laminaria Japonica processing.The extraction technology was optimized when crude yield and content of alginate taken as indicators through orthogonal test.The crude sodium alginate EA and the parts obtained by low concentration of ethanol precipitation was furtherpurifiedby D301-Tweak-base exchangeanionresin andfour fractions were obtained.The four fractions molecular weights were estimated by Sephacryl S-300 gel chromatography,andtheir structuralcharacteristicswere analyzedby IR spectrumandHPLC.The resultsandconclusionswereasfollows: 1、The contents of moisture,ash,protein and fat in Laminaria Japonica were 93.9%,11.86%, 8.70% and 0.2% respectively. An orthogonal test was designed to evaluate the optimum factors.Theresultsby orthogonaltest showedthatthe optimumextractionconditionwas as follows:extractiontimeof4.5hours,ratioofsolidtoliquidof 1:40,Na CO concentrationof2 3 3.5%andextractiontemperatureof70 ThecrudeyieldofsodiumalginateAextractedwas ℃. 44.07%withthecontentof80.99% 2、The D301-T weak-base exchange anion resin was comfrimed to be the best resin for the separationandpurification ofthe crudeproduct EAandAillustratedbythe staticadsorption anddynamicelutionexperiments.TheseparationparametersofD301-Tweak-baseexchange anion resin were determined as: pH of 7.5, velocity of flow of 1.0 mL/min, NaCl concentrationof0-2.5mol/L.Fourfractions,AF1 AF2 EF1andEF2wereobtainedwiththe 、 、 recovery yields of19.33% 63.33% 9.69% and 70.00%,and sodium alginate content of 、