sni模型大鼠脊髓背角n_甲基_d_天冬氨酸受体和降钙素基因相关肽的表达.pdfVIP

　2008 5 　25 　5 　J Clin Res, May 2008, Vol 25, №5 ·789 · ·论著 · SNI N--D- ＊ 1 1 2 1 王懿春 　王明德 　郭曲练 　赵江洪 (1.湖南省肿瘤医院麻醉科,湖南　长沙　410013;2.中南大学湘雅医院麻醉科,湖南　长沙　410008) 　　[]【目的】观察坐骨神经分支选择结扎切断模型(Spared nerve injury model, SNI)痛阈 脊髓背角 N-甲基-D-天冬氨受体(NMDA R)、降钙素基因相关肽(CGRP)表达的变化。【方法】健康雄性SD 大鼠27 只, 体重220～280 。 随机分为3 组(n =9)。 对照组(C 组);假手术组(sham 组);坐骨神经分支选择结扎切断 模型组(SNI 组)。在模型制作前 1 d 和模型制作后14 d 内,每组均进行疼痛行为学观察。所有组别均在模型 制作后d 、d 、d 天观察行为学改变后分批取材(每批 3 只),用免疫组化法观察大鼠L 节段水平脊髓背角 2 7 14 5 NM DA R 和CGRP 的表达。【结果】与C 组和sham 组比较, SNI 组在SNI 术后均出现机械性异常疼痛痛阈降 低(P0.01), 但热刺激后爪退缩潜伏时间无统计学意义(P0.05);同时SNI 组大鼠脊髓背角 NMDA R 和CGRP 免疫阳性产物数量和表达增加(P0.01)。【结论】SNI 模型大鼠出现典型的疼痛行为学特征时, 其脊髓背角NMDA R 和CGRP 表达也明显增强, 并且脊髓背角 NMDA R 和CGRP 的变化趋势大体相同。 　　[] 　脊髓;　D-天冬氨酸;受体;　降钙素基因相关肽;大鼠 Changes of Nervronal N-methyl-D-aspartate Recaptors and Calcitonin Gene-related Peptide Ex- pression in the Spinal Dorsal Horn Neuron Following Spared Nerve njury of Sciatic Nerve in Rats 　　　WA N G Yi-chun, 　GUO Qu-lian, 　WAN G Ming-de, et al (Dep artment of Anesthesiology, Hunan p rovince umor Hosp ital, Changsha Hunan 4100 13, China ) 　　[Abstract]【Objective】T o investi ate expression of N-methyl-D-aspartate recaptors(NMDA R)and Calci- tonin ene-related peptide (CGRP)in the spinal dorsal horn neurons induced by spared nerve injury of sciatic nerve.【Methods】twenty-seven healthy male Spra ue-Daw ley rats (220 ～280 )w ere random ly devided into three roups(n=9):control roup (roup C), sham roup (roup sham), spared nerve injury model roup ( roup SNI).These rats in roup SNI which received a lesion of two of the three terminal branchesof the sciatic nerve(tibial and common peroneal nerves)leavin the remain