铜胁迫下硫对玉米叶片叶绿素及MDA含量的影响.pdfVIP

~比 t.ι2013 ， 22(5) :25-29 Acta Agriculturae Boreali-occidentalis Sinica 铜胁迫下硫对玉米叶片叶绿素及 MDA 含量的影响 孔大为1 ， 2 ，贾 蓉2 ，周莉娜2 (1.天津市塘沽环保监测站.天津塘沽 300450; 2. 西北农林科技大学资源环境学院，陕西杨凌 712100) 摘 要 以农大 108 号玉米为供试材料，通过室内盆栽方法，测定 Cu( +Cu) 和 S(+S) 处理及二者共同处理 (+Cu+S)下玉米苗期和拔节期生物量、叶片 SPAD 值及丙二M(MDA)含量变化。结果表明，不同处理在玉 米苗期和拔节期的生物量分别表现为 CK十S 十Cu十S十Cu 及十SCK十Cu 十S十Cu ，说明铜污 染对玉米生长有明显的抑制作用，而增加硫营养可减轻铜对植株的毒害。玉米苗期不同处理间叶绿素含量差 异显著。在拔节期，+Cu 处理对 SPAD 值的影响显著增加，但补充硫营养后可明显减小铜对叶绿素的抑制作 用。苗期铜对ot 片 MDA 含量的影响显著，可导致 MDA 含量增大，说明铜胁迫可引起膜质过氧化程度加剧， 其产物 MDA 的积累增加;+S 与 CK 处理间变化不明显，但十Cu十S 较+Cu 处理的 MDA 的积累明显减少， 说明硫营养体现出一定的抵御铜胁迫的作用。 关键词 硫营养;铜胁迫;SPAD;MDA; 玉米 中图分类号 S513 文献标志码 A 文章编号 1004-1389(2013)05-0025-05 Effect of Sulfur on the Content of Chlorophyll and 岛1DA of 岛1aize under Copper Stress KONG Dawei 1.2 ,]IA Rong2 and ZHOU Lina2 (l. Tianjin Tanggu Environmental Monitoring Station. Tianjin 300450. China; 2. College of Nature Resources and Environment. Northwest A.F University , Yangling Shaanxi 712100 , China) Abstract Taking maize cultivar Nongda 108 as the plant materia l, an indoor pot experiment was con ducted to investigate the changes of biomass , SPAD and MDA during the maize seeding and jointing stage with sulfate under copper stress. The results showed that the biomass was in the order of CK 十S+Cu十S+Cu ìn the seedìng stage and +SCK十Cu十S十Cu in the joìntìng stage , ìllus- trating the significant inhibition of copper pollution on maize growth and the protective effect of sulfur nutrition on copper pollution. The difference of SPAD among the tested treatments during the seed ling stage was significant. In the jointing stage , the addition of copper resulted in a significant incr