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绿僵菌和白僵菌防蝗菌株的分子鉴定及室内致病力测定论文
Abstract
The entomopathogenic fungi Metarhizium and Beauveria have been developed
into different forms of fungal insecticides, and widely used in the biocontrol of insect
pests, Currently, There are several fungal insecticides products have been developed
to control locust, but the control effects were still unsatisfactory. Therefore, selection
of entogmopathogenic fungi with high virulence has been considered to be the basis
of biological control of locusts. In this thesis, firstly, different genotypes of fungal
strains was classified through DGGE method. Secondly, for accurate identification of
the strains, EF sequence-based PCR amplification and sequencing were conducted,
and phylogenetic tree was constructed for each of the strains. Thirdly, virulence of
strains was determined and selected through two bioassay methods of dipping and
feeding. Finally, biological characteristics of the selected highly pathogenic strains
were measured for good production traits. The main results were as follows:
1. The molecular identification of strains.
(1) Prelimary classification of the strains using DGGE method. the results of
DGGE profiles show that the tested 27 strains of Beauveria bassiana could be
classified to 6 genotypes, including A genotype: Bb854, B genotype: Bb1028, Bb6,
Bb860, Bb125, C genotype: Bb292, Bb654, Bb1179, Bb2270, Bb321, Bb322, Bb458,
Bb 859, Bb1372, Bb1843, Bb2153, Bb2157, Bb2248, Bb2250, Bb2251, D genotype:
Bb1519, Bb2253, Bb1906, Bb1975, E genotype: Bb1062, Bb2268, F genotype:
Bb324.
(2) Molecular identification of the fungal strains. 6 Beauveria spp. strains were
selected from the 6 genotypes, with one strain from every genotype. Together with the
other 4 stra
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