聚(NIPAAM-EDMA)和双苯基杂化整体柱的制备及应用.pdfVIP

摘 要 摘 要 高效液相色谱法（HPLC ）已经是分析领域重要的分析方法，色谱柱是HPLC 分析 过程中的核心。整体柱是一种新型的色谱柱，具有高效、低压、通透性好等优点，经过 功能化处理，色谱柱还可以制备成功能多样化的柱体。目前，整体柱已经成为色谱界研 究的重点和热点，其发展对分析、分离创新具有重大意义。 首先，本论文介绍整体柱的研究进展。 其次，以 N- 异丙基丙烯酰胺（NIPAAM ）为功能单体，乙二醇二甲基丙烯酸酯 （EDMA ）为交联剂，偶氮二异丁晴（AIBN ）作为引发剂，制备了一种新型整体柱材 料。对整体柱结构进行表征，以其作为富集柱，C18 柱为分析柱，对尿样中氢化可的松 进行富集分析。结论表明，该整体柱能够除去尿样中的生物大分子物质及其他成分的干 扰，对激素类药物有较好保留，可以实现尿样中激素药物快速、简便、有效的富集分析。 最后，以 γ-氨基丙基三乙氧基硅烷（HG-550 ）作为扩链剂与环氧树脂反应形成带 有双苯基的有机长链双硅烷，长链双硅烷再与四乙氧基硅烷（TEOS ）脱水缩合形成带 有双苯基、具有一定孔结构和机械强度的整体柱。新型整体柱作为固相萃取柱用于尿液 中倍他米松的富集，然后通过C18 柱进行了分析。 关键词 聚合物整体柱 有机-无机杂化整体柱 富集 分析 I Abstract Abstract High performance liquid chromatography (HPLC) has been an important analysis method, and column is the core of the HPLC. The monolith column is a new kind of column, which has the characteristic of high performance and low pressure as well as permeability, moreover, can be modificaed to different function column. So monolith column has been the emphasis and hotspot in the field of the HPLC. First, this paper reviewed the research progress of the monolith column. Second, a new monolithic column was prepared with N-isopropylacrylamide (N-IPAAM) as the monomer and ethyleneglycoldimethacrylate (EDMA) as the cross-linker. Then the structure of monolith column has been charactered. Then the monolith column was used to enrich hydrocortisone in urine sample, and then linked the C18 column for analysis. The results showed that this kind of monolith column could be used to extract the hormone drug and remove the biomacromoleculer in urine samples. In all, it is a fast, simple, effective method for assaying and analysis the horm