茶氨酸对咖啡碱引起的小鼠中枢兴奋的抑制作用.pdfVIP

group of mice was given each with 1mg/kg, 3mg/kg, 15mg/kg, 30mg/kg, 80mg/kg of theanine and 5mg/kg of caffeine at the same time for 7 days. After the last does, recording the times of free movement at 30min, 60min, 90min, 120min, 150min, 180min were recorded. Results showed that intake of caffeine alone enhanced the spontaneous activities of mice. By administration of theanine and caffeine at the same time, the spontaneous activity of mice induced by caffeine was inhibited in dose-dependent manner. In this paper, the influence of theanine on the metabolism of caffeine in blood of mice was also investigated. As the reference group, mice was given 5mg/kg of caffeine. The test group of mice was given each with 1mg/kg, 3mg/kg, 15mg/kg, 30mg/kg, 80mg/kg of theanine and 5mg/kg of caffeine at the same time for 7 days. After the last does, 0.2mL of blood was extracted form mice’s eyes at 30min, 60min, 90min, 120min, 150min, 180min, respectively. Contents of caffeine in mice blood was measured by HPLC. Results showed that theanine can affect the metabolism of caffeine in blood of mice. Caffeine was delayed to be absorbed into blood before 60min, and the digesting of caffeine in blood of mice was extend before 180min in dose-dependent manner. Key words Key words KKeeyy wwoorrddss: Camellia sinensis, Theanine, Caffeine, Spontaneous activity, Mice, Inhibit IV 1文献综述 1.1 前言 茶(Camelliasinensis)的发现和利用已经有几千年的历史，最初是作为药品 [1,2] 使用，后来发展成饮品，现在茶已成为仅次于水的世界流行饮料之一 。茶叶 按照加工工艺的不同可分为六大类：红茶、绿茶、青茶、黄茶、白茶和黑茶。其 中绿茶由于在加工过程中采用了高温杀青使酶失活，因而保留了茶鲜叶中大部分 [3] 的有效成分 。 700 茶叶中已经被分离、鉴定的化合物有 多种，包括初级代谢产物—糖类、 蛋白质、脂肪和茶树中的次级代谢产物—生物碱、多酚类、茶氨酸、芳香物质、 [4] 皂甙、色素等 ，这些次生代谢产物大多都具有独特的保健功能。 (N- -γ-L- N-ethyl-γ-L-glutamine Theanine) 茶氨酸 乙