RP-HPLC法分离异黄酮中移动相条件的优化.pdfVIP

Chin. J. Chem. Eng. , 15(2) 291 … 295 (2∞7) RESEARCH NOTES Optimum of Mobile Phase Condition for Resolving Isoflavones in RP翩HPLC ZHENG Jinzhu(郑金珠) and ROW Kyungho(1ìi敬臭)* Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, 253 Yonghyur卜Dong， N缸n幡Ku， Incheon402-751 , South Korea In reversed翩phase high performanωliquid chromatography 伺机HPLC)， the mobile phase condition for eight isoflavones (daidzin, glycitin, genistin , 6-o-acetyl daidzin , 6-o-malonyl genistin, daidzein, gly- mistein) was optimized using the HCI (High-Purity Separation Laboratory, [ Inha University) program software. The optimum composition of mobile ph~ lavones was obtained.ηle elution profiles were calculated by the plate 阳明 facωr， ln k口A+BF+cr， where F was the volume percentage of acetonitrih first mobile phase composition was water with 0.1 % AAlacetonitrile wíth 0.1 %AA (88%112% , by vol- followed at 9min later by the second composition of mobile phase which was step-chang时 to 85%/15%, at the third composition which wωstep-chang叫 to 73%127%, at 30min when it was changed to 659毛135% it was maintained in isocratic mode to the end of the run time at 50min. Although , using 8t叩 gradient separate 出e isoflavones , the calculated and experimented data were not achieved very good agreement, we estimate the closed retention time before experiment. And the agreement betwωn the experimental data and values was relatively good using isocratic separation for eight isoflavones, but the retention time is HCI progr，创n， optimum, separation conditi