尼罗罗非鱼Orexin前体基因克隆、.PDF

2011Jun. 32(3): 285−292 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI 10.3724/SP.J.1141.2011.03285 Orexin 1,2,# 1, # 1 1 1 1,* , , , , , (1. , 510275; 2. , 454000) RT-PCR cDNA (rapid-amplification of cDNA ends, RACE), (Oreochromis niloticus)RNA Orexin cDNA cDNA 648 bp, 423 bpOrexin 140 , 37 43 Orexin-A28 Orexin-B 32 Real-time PCR Orexin , 18 Orexin , ; , Orexin ; 246 8 d , Orexin , 4 d , Orexin ; Orexin; ; ; Q78; Q959.483 A 0254-5853-(2011)03-0285-08 Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus) 1,2,# 1,# 1 1 1 1,* CHEN Wen-Bo ,WANG Xin ,ZHOU Ya-Lu ,DONG Hai-Yan , LIN Hao-Ran , LI Wen-Sheng (1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals , School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; 2. Department of Biology, School of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, China) Abstract: We cloned the full length of tilapia prepro-orexin cDNA using RT-PCR and rapid-amplificatio