螺旋藻多糖含量测定(国外英文资料).docVIP

螺旋藻多糖含量测定(国外英文资料) The determination of spiral-polysaccharide content Update date: 2009-12-21 click: juyingluo yongming yan luan cao Feed by phenol - sulfuric acid method for content determination of the helix alga, method is simple, accurate, the average recovery was 99.71%, the coefficient of variation of 1.479%. Keyword spirulina; Polysaccharides; Content determination The deterination of Polysaccharides in Spitulina Maxima Zhu Ying, Luo Yongming, Yan Xiluan, et al C College of TCM, Nanchang 330006 AbstractThe deterimination of polysaccharides in spitulina maxima was made by a simple and precious method of phenol - sulphuric acid. The average rate of recovery is 99.71% and variation coefficient is 1.479%. Key words Spitulina maxima, Polysaccharides, Deterimination Spirulina (Spitulina Turpin) belong to the original nuclear plants cyanophyta, cyanophyceae, period of colonization, oscillatoria families, which contains rich nutrients and radiation resistance, antitumor, and adjust the immune function of halogen resistant algae. In recent years, studies have shown that the physiological active substances in spirulina, which are resistant to tumor and immune regulation, are polysaccharides, [1]. To this end, we use phenol - sulfuric acid method to determine the content of polysaccharide. 1 instrument and medicine Type 721 spectrophotometer (Shanghai), and 5% phenol solution (phenol, 100 g, adding aluminium 0.1 g and 0.05 g, sodium bicarbonate, distillation, collect 182 ℃ fraction, say take 10 g, 190 g of water, brown bottle in the freezer), Spirulina powder (provided by the nanchang bo source of foreign trade companies, varieties for maximum Spirulina Spirulina maxima). 2 experiment method [2] 2.1 polysaccharide extract: take spirulina powder 100 g, with industrial ethanol water bath reflux extraction twice, filtering, residue left enamel plate of water bath with ethanol, with eight times, six times, four times the amount of water cook for 1 h, respectively 40 min, 30