甘草渣中黄酮提取物质量标准探究.docVIP

甘草渣中黄酮提取物质量标准探究 　　摘要：目的 建立甘草渣中黄酮提取物的质量标准，为甘草渣中提取的总黄酮提供质量控制的方法及依据。方法 采用薄层色谱法将提取物与甘草苷及甘草对照药材对比鉴别，采用紫外分光光度法测定总黄酮含量，EDTA二钠滴定法测定钙离子含量。结果 供试品色谱中，在与对照药材色谱相应的位置上显相同颜色的斑点，在与对照品相应的位置上显相同颜色的荧光斑点。紫外分光光度法测定甘草苷在3.32～9.96 ?g/mL范围内线性关系良好（r=0.999 7），吸光度在0.3～0.7之间，平均加样回收率为102.41%（RSD=3.22%），3批甘草渣黄酮提取物中总黄酮平均含量为8.82%，钙离子平均含量为0.107%（RSD=3.61%）。结论 本方法重复性好、灵敏度高、结果准确，可用于甘草渣中黄酮提取物的质量控制 关键词：甘草渣；黄酮提取物；质量标准 DOI：10.3969/j.issn.1005-5304.2017.04.017 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2017）04-0067-04 Study on Quality Standard of Flavone Extracts in Licorice Residue LIU Xiao-shuan1， LI Xi-xiang1， XIAO Zheng-guo1， LI Ji-wen1， LUO Yan-yan2 （1. Pharmacy Department of Gansu Provincial Hospital of TCM， Lanzhou 730050， China； 2. Pharmacy College of Gansu University of Chinese Medicine， Lanzhou 730000， China） Abstract： Objective To establish the quality standard of flavone extracts in licorice residue； To provide the methods and basis for the quality control of total flavonoids extracted from licorice residue. Methods TLC was applied to compare the extracts with liquiritin and licorice； UV-spectrophotometry was used to measure the content of total flavones； EDTA-2Na titration was used to determine the content of calciumion. Results In the chromatogram of the test product， the spots of the same color were displayed on the corresponding position of the control medicinal material， and the fluorescent spots of the same color were displayed on the corresponding position of the control product. UV-spectrophotometry showed glycyrrhizin had a good linear relationship in the range of 3.32?C9.96 ?g/mL （r=0.999 7）， the absorbance between 0.3 and 0.7. The average recovery rate was 102.41% （RSD=3.22%）， and the average content of total flavonoids in three batches of flavonoids extract was 8.82%. The average content of calciumion was 0.107% （RSD=3.61%）. Conclusion This method has good repeatability， high sensitivity and accurate results， and can be used for the quality control of flavonoids in licorice r