2号、18号染色体微卫星多态性与甲状腺癌的关系论文.docVIP

　　2号、18号染色体微卫星多态性与甲状腺癌的关系论文 翟瑜 苏力 王春城 檀碧波 脱红芳 郭贵军 暴雷 郭怀斌 【摘要】 目的 探讨特定位点微卫星DNA序列不稳定性(MSI)及杂合性缺失(LOH)与人甲状腺癌发生、临床病理特征及预后的关系。方法 选取THRA1、D2S123、D11S912、BAT26四个位点，应用聚合酶链反应(PCR)和变性聚丙烯酰胺凝胶电泳技术，对60例人甲状腺癌患者的MSI及LOH表达情况进行研究。结果 THRA1位点MSI检出频率为43.3%，D2S123为36.7%；甲状腺滤泡癌中D2S123检出率为100%，未检测到BAT26；D18S58的检出率为26.7%；BAT26在甲状腺癌中检出率为6.7%；LOH 的检出率分别为33.3%、26.7%、23.3%和16.7%。术后随访5年，MSI/LOH阳性的甲状腺癌较阴性者生存期更长（P＜0.05）。 结论 在2号和18号染色体中检测到微卫星阳性率较高；D2S123位点MSI与滤泡型甲状腺癌相关性有统计学意义；D18S58位点MSI的阳性率与高龄患者、晚期肿瘤密切相关；BAT26在甲状腺癌中检出率最低。MSI/LOH导致基因组不稳定，在甲状腺肿瘤发生过程中发挥作用。MSI/LOH阳性的甲状腺癌患者较阴性者生存期更长。 【关键词】 甲状腺癌；MSI；PCR；肿瘤 【Abstract】 Objective To investigate the relationship beticrosatellite instability (MSI) and the loss of heterozygosity (LOH) in thyroid carcinoma and the occurrence, clinic pathological features and prognosis. Methods THRA1, D2S123, D11S912 and BAT26 site point ide gel electrophoresis (PAGE). The expressions of MSI and LOH in 60 patients a easured and the patients 5year survival rate ost frequent MSI or. In follicular cancer, MSI in D2S123 occurred at a frequency of 100% (12/12), 0% in BAT26 and 26.7% in D18S58. BAT26, portant marker in colorectal cancer, displayed the loors 6.7% (4/60). The expression rate of LOH survival (P=0.007). Conclusions The high frequency of MSI can be detected at both 2p and 18q loci. It is identified that there are correlations betosome and clinical pathologic characteristics. The link beta is calculated to be statistically significant. The correlation betinal tumor is significant. This finding of the loical defects governing the spectrum of MSI in thyroid and colorectal cancer are different. MSI/LOH leads to the instability of the genome, has the notable significance in occuring of the thyroid tumors. Patients survival. 【Key a; MSI; PCR; Tumor 甲状腺癌来源于上皮滤泡细胞或滤泡旁C细胞〔1〕，目前多根据患者年龄、肿瘤大小和病理特征确定潜在的风险.freelicrosatellite，MS)、微卫星不稳定性(microsatellite instability，MSI)和杂合性缺失(loss of heterozygosity，LOH)，是继癌基因和抑癌基因之后，研究肿瘤发生、发展和预后方面的又一热点。MSI最早发现于遗传性非息肉性结肠癌(Hereditary Nonpoly