MCM5和p16蛋白在肺腺癌组织中的表达及其临床意义论文.docVIP

　　MCM5和p16蛋白在肺腺癌组织中的表达及其临床意义论文 【摘要】 目的 检测微小染色体维持蛋白5(MCM5)、p16蛋白在肺腺癌组织中表达的改变及其在癌发生发展中的作用。方法 采用免疫组织化学SP法检测60例肺腺癌、30例支肺脓肿等非癌病变旁正常肺组织中MCM5、p16蛋白的表达情况。结果 60例肺腺癌组织中MCM5蛋白的阳性表达率为100%，与正常对照组的表达差异有高度显著性（P＜0.01）。肺腺癌组织中p16蛋白阳性表达率为71.67%（43/60），显著低于100%阳性表达的正常对照组（P＜0.01）。肺腺癌组中MCM5、p16蛋白的阳性表达与组织学分级有关（P＜0.01）；癌组中MCM5蛋白的阳性表达与淋巴结转移有关（P＜0.01），而p16蛋白的阳性表达与淋巴结转移无关（P＞0.05）。MCM5与p16蛋白的表达与肺腺癌的年龄、肿瘤大小、组织学类型及TNM分期无关（P＞0.05）。MCM5蛋白的阳性表达与p16的表达呈负相关（r=-0.4920，P 0.01）。结论 MCM5蛋白的高表达可能涉及了人肺腺癌的发生、发展过程，MCM5蛋白的高表达与癌细胞的增殖、侵袭和转移有关，MCM5的检测可作为评价肺腺癌癌细胞的增殖状态、预测肺腺癌临床预后、指导临床治疗的重要生物学指标。p16基因改变在肺腺癌的发生中起重要作用，p16蛋白是肺腺癌早期诊断的有用指标。 【关键词】 肺肿瘤 MCM5 p16 免疫组织化学 Abstract: Objective To outline the significance of minichromosome maintenance proteins 5 (MCM5) and p16 protein expression and to evaluate their correlations or progression in lung adenocarcinoma. Methods The SP immunohistochemical technique a, and 30 cases of normal lung tissues of non-cancerous lung disease (bronchial pulmonary abscess, etc). Results The expression of positive MCM5 in 60 cases of lung adenocarcinoma al lung tissues (P 0.01). The expression of positive p16 in lung adenocarcinoma al lung tissues (100.00%) (P 0.01). The expressions of positive MCM5 and p16 protein a (P 0.01), MCM5 protein positive expression ph node metastasis in lung adenocarcinoma (P 0.01) , and p16 protein or size, histological classification and TNM stage (P＞0.05). MCM5 ay play an important role in the pathogenesis and development of lung adenocarcinoma. The high expression of MCM5 etastasis of lung adenocarcinoma. The detection of MCM5 protein may be a useful predictor of cell proliferation, biological behavior and prognosis of lung adenocarcinoma. p16 gene mutation plays an important role in pathogenesisof lung adenocarcinoma, and p16 protein may be an early event. Key s; MCM5; p16; immunohistochemistry 肺癌是人类常见的恶性肿瘤之一.freelinichromosome maintenance proteins，MCM）参与了DNA复制起始的调控，MCM5是MCM家族成员之一，近年国外已有研究表明其可作为不典型增生和肿瘤的生物学标记物［1］。p16基因编码的p16蛋白为细胞周期的一种负性调节因子，可与细胞周期素D竞争结合细胞周期