人子宫内膜异位症在位和异位内膜细胞原代培养及形态学观察论文.docVIP

　　人子宫内膜异位症在位和异位内膜细胞原代培养及形态学观察论文 俞超芹，石书芳，刘玉环，王瑞霞，宋艳华，俞瑾 【关键词】 子宫内膜异位症 ［摘要］ 目的：探讨子宫内膜异位症（endometriosis, EM）异位子宫内膜细胞原代培养方法，并比较EM在位及异位子宫内膜细胞与正常对照在位子宫内膜细胞形态的差异。方法：采用改良EM细胞原代培养方法，免疫细胞化学法鉴定细胞类型.freelary culture and morphologic observation of eutopic and ectopic endometrial cells from patients ehiosis ABSTRACT Objective: To explore the method of primary culture for endometriotic cells and to find out the differences in morphological manifestations among endometriotic cells and eutopic endometrial cells sampled from patients etriosis and endometriosisfree en. Methods: Endometriotic and eutopic endometrial cells odified method of primary culture. The endometriotic cell types icroscopes. Results: The success rates for culture of eutopic endometrial cells from endometriosisfree en and patients etriosis etriotic cells etriotic glandular cells ilar to those of eutopic endometrial glandular cells from endometriosisfree en and patients etriosis. The chromatin anifold and the nucleus ented in the endometriotic glandular cells. The endometriotic stromal cells aller than the eutopic endometrial stromal cells from endometriosisfree en and patients etriosis. Many tiny villi and protuberances on plasma membrane could be seen in the endometriotic stromal cells. Conclusion: The success rate for culture of endometriotic cells can be elevated through improving the method of primary culture. The ultrastructures of endometriotic glandular and stromal cells are obviously different from those of eutopic endometrial glandular and stromal cells from endometriosisfree en and patients etriosis. KEY ）是子宫内膜间质细胞和腺上皮细胞种植于子宫腔以外的雌激素依赖性疾病，具有侵袭性生长、广泛种植、易复发等恶性肿瘤样特性。虽然EM的发现已有一百多年的历史，但其病因尚不十分清楚，亦无有效的治疗方法。因此，EM是目前妇科学界研究的热点之一。已有的大量研究表明，异位子宫内膜间质细胞与腺上皮细胞的功能及相互作用不同于在位内膜［1,2］，而原代培养细胞由于刚离开机体，因此能较好地反映体内细胞的生物学特性，故原代培养已成为EM体外研究的一个重要方法。实践表明，在位和异位内膜间质细胞及腺上皮细胞的分离培养成功率并不高，尤其是异位内膜细胞培养的成功率较低，故本研究对EM在位内膜细胞及异位内膜细胞的分离培养方法进行了探索，观察正常子宫内膜细胞、EM在位内膜细胞及EM异位内膜细胞之间形态学的差异，从细胞形态学角度研究EM的发病机制，以期为EM的研究提供有价值的体外细胞模型。 1 材料与方法 1.1 材料 1.1