VEGF在胰腺缺血再灌注损伤中的作用论文.docVIP

　　VEGF在胰腺缺血再灌注损伤中的作用论文 汤礼贵，宋胜江，安慧敏，蔡小芳，朱华 【摘要】 目的 ：探讨血管内皮生长因子(VEGF)在缺血再灌注损伤(IRI)胰腺组织中的表达及其意义。方法：将雄性SD大鼠32只随机分为4组(n =8)。A组为对照组;B组、C组和D组分别通过钳闭大鼠腹腔干和肠系膜上动脉15、30和60 min造成胰腺缺血，然后再灌注6 h建立缺血再灌注损伤模型。对各组胰腺组织进行湿干质量比(ethods: Thirty tale SD rats ly divided into four groups (n =8). Group A served as control group， Groups B， C and D ins， 30 mins and 60 mins of ischemia respectively by clamping of celiac artery and superior mesenteric artery， then released for 6 hours to produce ischemia reperfusion injury model. Rats oved for histological injury evaluation and detection of munohistochemical staining of VEGF. Results: The expression of VEGF in pancreas increased after ischemia reperfusion injury. There ong the islet cells，stromal vascular epithelial cells，acinar and duct epithelial cells of pancreas (P 0.01). Pathological score and munohistochemical staining of VEGF in the islet cells (P 0.01) and shoal vascular epithelial cells (P 0.01). Conclusion: The edema of the pancreas caused by ischemia reperfusion injury may be related to the increased expression of VEGF. Key ia/reperfusion injury;pancreas 缺血再灌注损伤(ischemia reperfusion injury，IRI)是引起胰腺移植后早期功能丧失的主要原因之一。研究发现血管内皮生长因子(vascular endothelial grog/kg腹腔注射麻醉。其中B组、C组和D组分别用血管夹阻断大鼠腹腔干及肠系膜上动脉15、30和60 min，然后再灌注6 h，脊椎脱臼法处死大鼠，取胰腺组织标本;A组为对照组，仅解剖显露大鼠腹腔干及肠系膜上动脉，6 h后取胰腺组织标本。 1.3 观察指标和检测方法 ①胰腺组织标本分成两份，其中一份用电子分析天平称湿重，置90 ℃电热干燥箱内烘烤72 h后再称干重，并计算胰腺湿/干质量比(eyer S, Thorn D,et al. Targeting vas- cular endothelial groicrovascular disturbances during is- chemia/reperfusion of the pancreasJ. Transplantation, 2006,82(4):543-549. 2 杨立新,黄俊,戢朝明,等. 急性胰腺炎血液流变性改变的动态 观测及与胰腺组织损害相关性研究J. 中国血液流变学杂志, 2000, 10(3): 132-134. 3 程若川,汤礼贵,兰丽琴.槐耳清膏诱导人直肠癌HR8348细胞 凋亡的实验研究J.中国肿瘤，2003,.freeleability factor enhances vascular permeability via nitric oxide and prostacyclinJ. Circulation,1998,97(1): 99-107. 5 Dvorak HF, Broar M, et al. Vascular perme- ability factor/vascular endothelial groicrovas- cular hyperpermeability, and angiogenesisJ. Am J Pathol, 1995,146(5)