中药提取物葫芦素B_PLGA微球的制备及处方工艺优化.pdfVIP

B- PLGA # 周丽莹 , 关 津, 聂淑芳, 潘卫三 (沈阳药科大学药学院, 沈阳市 110016) R2842; R944 9 A 100 1- 0408(2009) 33- 2588- 05 目的: 制备中药提取物葫芦素( Cuc) B- 乳酸- 羟基乙酸共聚物( PLGA) 微球方法: 应用改良的乳化溶剂挥 法制备微 球; 采用星点设计- 效应面法优化制备工艺, 以聚乙烯醇(PVA) 浓度和投药比为自变量, 微球的产率( Y 1)载药量( Y 2) 包封率 ( Y 3)粒径( Y 4)24 h 累积释放量( Y 5) 为指标, 进行多元线性回归和二次多项式拟合; 改良的直接释药法考察微球的体外释放情 1 2 3 4 5 况结果: Y Y Y Y Y 二次多项式方程拟合效果较好, 较优的工艺条件为PVA 浓度00 14, 投药比0066 5制得的微球形 态圆整, Y 1Y 2Y 3 Y 4Y 5 分别为 79 9%783 %805%5618 m698%体外释放35 d 的累积释放量为8673%结论:制 备的CucB- PLGA 微球满足了长效缓释的要求, 所建立的模型预测性良好 葫芦素B;乳酸- 羟基乙酸共聚物;微球; 星点设计; 效应面; 体外释放 Pre aration and O timization of Formulation of PLGA Micros heres Containin Chinese Herbal Extract p p p g Cucurbitacin B Z OU Li_ in , GUAN Jin,NIE Shu_fan , PAN Wei_san(Colle e of Pharmac , Shen an Pharmaceutical U y g g g y y g niversity, Shenyang 110016, China) AB TRACT OBJECT IVE: T o prepare Polylactic- co- glycolic acid) (PLGA) microspheres loaded with Chinese herbal ex tract cucurbitacin BMET ODS: Cucurbitacin B loaded by PLGA microspheres were prepared by modified emulsification- solvent evaporation methodCentral composite design- response surface method was applied to optimize the formulation with PVA concentration and ratio of dru to ol mer as inde endent variables, and with ield of micros heres( Y 1), dru loadin g p y p y p g g amount ( Y 2) ,enca sulation efficienc ( Y 3), mean article diameter ( Y 4) , and thecumulative ercenta e of the dru releasein p y p p g g 24 hr( Y 5) as indexes to conduct multi le linear re ression and second- order ol nomial e uation fittin In vitro release test