草甘膦对转EPSPS-G6基因棉花种质系配子育性的影响.pdfVIP

花 学 报 Cotton Science 20 13 25 渊2 冤院115~ 120 甘膦对转 6 基因棉花种质系配子育性的影响 梅磊 陈进红 何秋伶 祝水金* 浙江大学农业与生物技术学院袁杭州310058冤 摘要院以转 6 基因的抗草甘膦棉花种质系为材料袁研究转基因抗草甘膦棉花植株现蕾后喷施不同浓 度草甘膦后的花器形态结构和配子育性的影响遥 结果表明袁苗期的草甘膦处理对转 6 基因的棉花种 质系植株生长发育无明显的影响袁但现蕾后可导致转基因抗草甘膦棉花种质系花冠变小袁花丝变短袁柱头变 长袁花药皱缩尧不能开裂袁呈雄性败育的特征曰高浓度 30 mmol 窑L-1冤草甘膦处理尤其明显遥 花粉粒育性鉴 结 果表明袁草甘膦处理15 20 d 后转基因植株的花朵雄配子即完全败育袁其中袁低浓度 20 mmol 窑L-1冤下完全败 -1 育的时间为20 d 袁高浓度 30 mmol 窑L 冤下完全败育的时间为15 d遥低浓度草甘膦处理的花粉粒的完全败育持 续时间约20 d 袁高浓度草甘膦处理完全败育的持续时间更长遥 不同间隔时间喷施草甘膦的试验结果表明袁间隔 20 d 喷一次草甘膦袁即可在首次喷药20 d 后保持完全不育遥 考虑到气候等因素袁转基因抗草甘膦棉花可在现 蕾初期喷施20 mmol 窑L-1 的草甘膦袁每隔 15 d 遇特殊气候可延长5 d 冤喷1 次,共喷4 次袁即可进行不去雄杂交 棉制种遥 关键词院草甘膦曰杀雄剂曰杂交制种曰不育机理 中图分类号院S562.03 文献标志码院A 文章编号院1002-7807 渊20 13冤02-0115-06 MEI Lei, CHEN Jin-hong, HE Qiu-ling, ZHU Shui-jin * ( 310058, ) The effect of glyphosate on floral organ morphology structure and gamete fertility of transgenic glyphosate-resistant cotton germplasms with 6 were studies, with the germplasms were treated with different glyphosate concentration start- ed from squaring stage. The results showed that there were not obvious damage on the growth and development of the transgenic germplasms by the treatment of glyphosate before squaring stage, but serious flower characters and male sterility, such as small flowers, short stigmas, wizened and indehiscent anthers etc., when they were treated with glyphosate after squaring stage espe- cially with the high concentration(30 mmol 窑L-1 ). Pollen fertility identification showed that male game