短季棉叶片早衰的比较蛋白质组学研究.pdfVIP

花 学 报 Cotton Science 20 13 25 渊2 冤院162~ 168 季棉叶片早衰的比较蛋白质组学研究 1 2 2 2 2 1,2* 卢超 张根连 范术丽 宋美珍 庞朝友 喻树迅 1. 西北农林科技大学农学院袁陕 杨凌712100 曰2. 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁 河南安阳455000 冤 摘要院选取短季棉品种中棉所10 号为研究材料袁在棉花叶片衰老过程中袁分别取30 d 袁40 d 和50 d 时的叶片进 行叶片全蛋白的提取袁利用双向电泳技术分析叶片衰老过程中的差异蛋白遥 考马斯亮蓝染色尧扫描得到双向电 泳图谱后袁利用软件ImageMaster 2D Platinum 7.0 分析差异蛋白遥 结果表明袁在这3 个时期里袁共有33 个蛋白 点表达水平显著变化曰 对选取的差异蛋白点进行MALDI-TOF-TOF 质谱鉴定袁 最终成功鉴定其中12 个蛋白 点遥 蛋白质组学分析表明衰老过程中院参与病虫害防御反应的蛋白6 个显著下调表 袁1 个上调表 曰参与光 合作用的蛋白2 个上调表 袁1 个下调表 曰参与信号转导的2 个蛋白均下调表 遥 关键词院棉花曰叶片早衰曰双向电泳曰蛋白质组学 中图分类号院Q816 文献标志码院A 文章编号院1002-7807 渊20 13冤02-0162-07 1 2 2 2 2 1,2* LU Chao , ZHANG Gen-lian , FAN Shu-li , SONG Mei-zhen , PANG Chao-you , YU Shu-xun (1. 712100, 曰2. 455000, ) Short-season variety CCRI 10 was chosen as the experimental material, and during leaf senescence, cotton leaves were collected at 30 d, 40 d, 50 d for whole protein extraction. The extracted protein was further separated by the two-dimensional electrophoresis technology. After coomassie brilliant blue staining and gel scanning, protein spots from all the replicate gels were compared and quantified using the Image Master 2D Platinum Software Version 7.0, and a total of 33 stained spots were found to have significant changes during the three developmental stages. These protein spots were then subj ected to MALDI-TOF-TOF analysis and finally 12 protein spots were successfully identified. Proteomic analysis revealed that during cotton leaf senescence: 6 proteins involved