奈米金探针应用於区分肺结核杆菌之方法论文.pdfVIP

Gold Nanopart cle Probe assai y for identification of Mycobacterium tuberculosis complex (奈米金探针应用於区分肺结核杆菌之方法) Yu-Tze Horng1 (洪育芝), Po-Chi Soo1* (苏伯琦) 1 Graduate School of Biotechnology and Bioeng neering, Yuan Ze University, 135i Yuan-Tung Road, Chung-Li 320,4 Taiwan, Republic of China （台湾 元智大学 生物科技与工程研究所） E-mail: pcsoo@.tw ; Tel: 886-3-4638800 ext. 2336 Abstract The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thus important in certain occasions. Many molecular diagnosis assays were developed that means the more advantages using molecular diagnosis than using conventional diagnosis in clinical specimens. In this study, we developed the diagnosis method that utilizes gold nanoparticles derivatized with thiol modified oligonucleotides that are designed to hybridize specifically complementary DNA targets. Two pairs of gold nanoparticles probes, GP-IS6110 and GP-rv3618, are designed to specifically hybrid with target DNA of MTBC and MTB strains, respectively. We use gold nanoparticles probes assay for simultaneously and directly detecting not only MTBC but also MTB from clinical sputum specimens. The results were compared with traditional culture and biochemical identification results together with patients clinical assessments. This assay showed the high sensitivity and high specificity for detection of MTB. This detection system shows great potential in clinical application.