氯化1-十六烷基-3-甲基咪唑对泥鳅胚胎的毒性效应分析.doc

离子液体[C16 mim]Cl对泥鳅胚胎及仔鱼的毒性研究 学院名称： 专业名称： 年级班别： 姓 名： 指导教师：   离子液体[C16 mim]Cl对泥鳅胚胎及仔鱼的毒性研究 摘 要 本研究以泥鳅的胚胎及仔鱼为试验材料，选择胚胎发育的个不同时期（、原肠胚中期、神经胚期、尾芽期和仔鱼孵出期）为开始处理时间，探讨了的毒性作用。结果表明：随着的浓度增加，泥鳅胚胎的孵化率下降，畸形率上升，具有明显的剂量－效应关系；随着的浓度增加，泥鳅仔鱼死亡率上升，处理组的仔鱼体长均短于对照组，具有明显的剂量－效应关系结论：对泥鳅胚胎及仔鱼的发育，有明显的抑制、致畸、致死作用，表现出明显的毒性作用。为 关键词 [C16 mim]Cl，泥鳅，胚胎发育，毒性 Study on Toxicity of [C16 mim]Cl on the Embryos and Larvae of loach （Misgurnus anguillicaudatus） Abstract In this paper, we used the embryos and larvae of loacMisgurnus anguillicaudatus ）for the test materials, choiced five different embryonic development periods ( Blastula stage, Mid-gastrulation, Neurula stage, tail bud stage and larval fish hatched period) as the beginning of the processing time, studied the toxicity effect of [C16 mim]Cl on loach. The results showed：With the increase in the concentration of [C16 mim]Cl, the hatching rates of loach embryos were declined and meanwhile its abnomal embryos rates were increased, had obvious dose - effect relationship. With the increase in the concentration of [C16 mim]Cl, the mortality ratio of loach larvae were increased. And the treatment groups were shorter than the control group, with significant dose - effect relationship. Conclusion: The Ionic Liquid (IL) of [C16 mim]Cl had inhibited the development, teratogenic, lethal effect on the loach embryos and larvae, showing obvious toxicity. Therefore, it is necessary to provide a scientific basis for the reasonable application amount of. [C16 mim]Cl. Keywords [C16 mim]Cl; Misgurnus anguillicaudatus; Embryonic development; Toxicity 前 言 离子液体(ILs)是一类完全由离子组成的室温下呈液态的新型化学试剂，因其不挥发、不易燃和热稳定等优势，具有广阔的应用前景，曾被认为是环境友好的“绿色产品” [1,2]。虽然离子液体无挥发性因而不会造成大气污染，但大部分离子液体是水溶性的，在合成和应用过程中，它们很可能通过诸如偶然的泄漏事件、化学工业的污水排放等途径进入地表水和地下水，从而对环境特别是水环境产生潜在的威胁[3,4]。对一些离子液体对发光细菌、大型蚤、斑马鱼和淡水蜗牛的毒性效应的研究发现，离子液体毒性的决定性因素是阳离子，毒性随阳离子取代基碳链的增长而增加[5-7]。因此，检测水体中的指标具有重要的现实意义。 泥鳅(Misgurnus anguillicaudatus)鲤形目、