内异康复栓对子宫内膜异位症大鼠异位和在位内膜雌、孕激素受体的影响论文.docVIP

　　内异康复栓对子宫内膜异位症大鼠异位和在位内膜雌、孕激素受体的影响论文 魏绍斌,曹亚芳,王毅,郭蓉晓,仝崇毅,谭亿民 【摘要】 目的 观察内异康复栓直肠给药对子宫内膜异位症大鼠异位和在位内膜雌激素受体(ER)、孕激素受体(PR)表达的不同影响，探讨其作用机理。方法 将子宫内膜异位症大鼠随机分为内异康复栓高、中剂量组及丹那唑组、丹莪妇康煎膏组、模型组、空白组,采用免疫组化SP法检测ER、PR的表达。结果 内异康复栓高、中剂量组能显著降低异位内膜ER、PR的表达(P 0.05),并对在位内膜下降的ER、PR水平有上调作用(P 0.05)。结论 内异康复栓可能通过抑制异位内膜细胞内ER、PR的合成而发挥治疗作用,且对在位内膜异常的ER、PR表达有一定的调节作用。 【关键词】 子宫内膜异位症；内异康复栓；雌激素受体；孕激素受体；大鼠 Abstract：Objective To explore the different influence of Neyikangfu suppository (NYKFS) on ER and PR in ectopic and eutopic endometrium of endometriosis model rats by treatment of rectal administration. Methods Healthy and sexual matured female SD rats etriosis model ly formed and iddle-dose groups of NYKFS, Danazol capsule group and Danefukangjiangao (DEFKJG) group, model group and normal control group. Expressions of ER and PR in ectopic and eutopic endometrium and endometrium of normal group easured by immunohistochemical SP staining method. Results High-dose and middle-dose groups of NYKFS could obviously loetrium of endometriosis model rats. High-dose and middle-dose groups of NYKFS could increase the expression of ER and PR in eutopic endometrium to certain extent (P 0.05). Conclusions NYKFS can inhibit the synthesis of ER and PR in cells of ectopic endometrium to treat endometriosis and have certain acmodation on abnormal expression of ER and PR in eutopic endometrium. Key etriosis；Neiyikangfu suppository；estrogen receptors；progesterone receptors；rat 子宫内膜异位症(EMT)是激素依赖性疾病,异位内膜的生长和维持依赖于周期性卵巢激素的刺激,需通过雌激素受体(ER)和孕激素受体(PR)发挥作用。异位内膜ER和PR的浓度不同于在位内膜且缺乏周期性变化1。以湿热瘀结立论的内异康复栓,.freelg,上海华联制药有限公司生产,国药准字批号050301。 1.3 试剂及仪器 一抗购自Santa Cruz Biotechnology,INC公司：ERα(MC-20)rabbit polyclonal IgG,200 μg/mL；PR(H-190) rabbit polyclonal IgG,200 μg/mL；二抗及相应试剂盒购自北京中杉生物技术有限公司。光学显微镜,型号：BH-2；数码摄像机,型号：TK-C1381EG；BIOMIAS图像分析系统。 1.4 造模 采用自体移植方法2建立EMT大鼠模型,2.5%戊巴比妥钠 (0.13 mL/100 g)腹腔内麻醉,待麻醉生效后,将大鼠固定在操作台上,腹部脱毛,常规消毒手术区皮肤,无菌操作,腹部正中纵切口,长约2 cm,开腹分离右侧子宫,结扎子宫两端及血管,切取右侧中段子宫。分离附着的脂肪和结缔组织后,将子宫纵形剖开,迅速分成约5 mm×5 mm大小的3段,分别种