蛋白质晶体学课件-8_944403252.pdf

Data Collection and Reduction Structure Factor Statistics Jiawei Wang School of Life Sciences, Tsinghua University Layout of Experiment 2013/11/21 Jiawei Wang jwwang@ 3 Bruker-Nonius mini-Kappa Goniometer 2 Theta -98 °～78 ° Omega -270 °~ 270 ° Phi 0 °~ 360 ° Kappa -175°~ 175 ° Distance 45 ~ 200 mm 2013/11/21 Jiawei Wang jwwang@ 4 Bragg’s law Ewald Sphere Real Space Reciprocal Lattice 2 d sinθ= λ 1/d=(2/λ)sin θ 2013/11/21 Jiawei Wang jwwang@ 5 As the crystal rotates, so does the reciprocal lattice As a reciprocal lattice point passes through the Ewald sphere, a diffracted beam is observed along the line from the sphere center to the reciprocal lattice point The part of the reciprocal lattice which intersects the sphere is projected on to the detector As the crystal rotates, each lattice point in turn passes through the sphere, and a spot is recorded on the detector. The appearance of diffraction images Reciprocal lattice points lie in layers (planes). Each plane intersects the sphere in a circle, and the spots 2013/11/21 Jiawei Wang jwwang@ 9 projected on the detector lie in ellipses Rotation/Oscillation Method If the crystal is rotated through a small angle, each circle is