叶酸降低高同型半胱氨酸大鼠主动脉内皮黏附分子VCAM论文.docVIP

　　叶酸降低高同型半胱氨酸大鼠主动脉内皮黏附分子VCAM论文 黎明,陈健,冯义柏,李裕舒,谷翔,史春志 【关键词】 高同型半胱氨酸 Folic acid reduces adhesion molecules VCAM1 expression on endothelium in rats ocysteinemia 【Abstract】 AIM: To investigate the effects of folic acid supplementation on the expression of adhesion molecules VCAM1 in aortic endothelium of rats ocysteinemia (HHCY) induced by ingestion of excess methionine (MET). METHODS: Thirty male SD rats（200±20 g）al diet, normal diet enriched by 17 g/kg MET and normal diet plus 17 g/kg MET and 0. 06 g/kg folate for 45 d. The levels of total plasma homocysteine (HCY) RNA in aorta of rats munohistochemistry and RTPCR. RESULTS: The highmethionine diet resulted in a significant increase in the plasma HCY levels ［(140.7±36.9) μmol/L vs (6.5±1.1) μmol/L］. The serum HCY levels ethionine plus folaterich diet than those in rats fed on the highmethionine diet ［(50.6±21.8) μmol/L vs (140.7±36.9) μmol/L, P 0.05］. The expression of adhesion molecules VCAM1 at protein and mRNA levels of rats fed on the highmethionine diet than that in control rats. The expression of VCAM1 at protein and mRNA levels of rats fed on highmethionine plus folaterich diet pared ethionine diet. CONCLUSION: A high methionine diet for 45 d is sufficient to induce HHCY. Folate supplementation to the rats fed on the highmethenine diet prevents the elevation of HCY levels in the blood and reduces the expression of adhesion molecules VCAM1 in aorta of rats ocysteinemia; adhesion molecules VCAM1; folate 【摘 要】 目的：探讨叶酸治疗对高蛋氨酸(MET)喂养所致的高同型半胱氨酸(HHCY)血症大鼠主动脉内皮黏附分子VCAM1表达的影响. 方法: SD大鼠30只随机分3组: 正常对照组(Control组), 高蛋氨酸组(MET组)和蛋氨酸＋叶酸组(MET＋Folate组), 每组10只，分别给予普通饲料，普通饲料加17 g/kg蛋氨酸，普通饲料加17 g/kg蛋氨酸和0.06 g/kg的叶酸，饲养45 d，测定血浆总同型半胱氨酸浓度(HCY)，分别采用免疫组织化学染色法及RTPCR法检测大鼠的主动脉VCAM1蛋白及mRNA表达. 结果：高MET组血浆HCY浓度明显高于Control组［（140.7±36.9) μmol/L vs (6.5±1.1) μmol/L, P＜0.05］，MET＋Folate组血浆HCY浓度明显低于高MET组［（50.6±21.8) μmol/L vs (140.7±36.9) μmol/L, P＜0.05］；在高MET组中.freelRNA水平均较Control组明显增加，而MET＋Folate组减少蛋氨酸饮食诱导的HHCY血症所致的主动脉内皮表达的细胞黏附分子VCAM1表达. 结论: 补