毛白杨油酸去饱和酶基因PtFAD2的克隆与表达分析_周洲.pdfVIP

43 7 Vol.43, No.7 2 0 0 7 7 SCIENTIA SILVAE SINICAE Jul., 2 0 0 7 PtFAD2 ＊ 周　洲　张德强　卢孟柱 (　 100091) 　:　, , EST , , , PtFAD2 cDNA ; RT-PCR PtFAD2 cDNA (GenBank :DQ31 788), cDNA 127 bp, 388 。 RT-PCR :P tFAD2 、、 , 4 ℃, 。 :　;;;PCR; :S718.4 ;Q943.2　　　:A　　　:1001-7488(2007)07-001 -0 Cloning and Expression Analysis of PtFAD2 Gene Encoding the Endoplasmic Reticulum Fatty Acid 18 ∶1 Desaturase in Populus tomentosa Zhou Zhou 　Zhang Deqiang 　Lu Mengzhu (Key Laboratory of Tree Breeding and Cultivation of S tate Forestry Administration 　Research nstitute of Forestry, CAF 　Bejiing 100091) Abstract:　The endoplasmic reticulum 18 ∶1fatty acid desaturase (FAD2)is to provide 18 ∶2 fatty acid required for the correct assembly of cellular membranes throughout the plant.In this study, a full-length cDNA clone of PtFAD2 gene (GenBank accession number:DQ31 788)was firstly isolated using the in silico cloning method in Pop ulus tomentosa .It is 1 27 bp in length and the open reading frame encodes a peptide of 388 amino acids, which match the known peptide sequences.The predicted amino acid sequence shows significant homology with those of other plant species, which contain typical domains owned by FAD2 proteins.The transcripts of PtFAD2 are equal in leaves, stem and roots using semi-quantitative RT-PCR.When the shoots were experienced the cold treatments, the transcripts of PtFAD2 isnt reduced in 24 h.This suggests that the PtFAD2 may be a structural expression protein and it isnt induced by low temperature.This study provides the basis for not only cloning and research of poplar fatty acid desaturase gene, but also the genetic engineering of plant fatty acid in the near future. Key words:　Popul