肺巨噬细胞移植治疗--培训课件.pptVIP

PMT of WT LysMGFP knock-in mouse25 BMDMs into KO mice followed by Ki67 immunostaining revealed PMT-derived cells replicated in vivo (Extended Data Fig. 3e-g). Ki67是与增殖相关的核抗原，Ki67标记G0期(非增殖期)以外的细胞，Ki67阳性细胞比例越高，处于增殖时期的细胞比例越高，肿瘤恶性程度越高。 GFP Ki67 GFP+Ki67 (e) Macrophage replication after PMT. KO mice received Lys-MGFP BMDMs by PMT and paraffin-embedded lung was immunostained for Ki67 one month or one year later (c)The percentage of Ki67+PMT-derived alveolar macrophages was 32.2 _+ 6.05% one month after PMT and declined to 11.29 _+ 2.2% by one year (Fig. 2c) (f) Ki67 staining of BAL cells from an un