反相高效液相色谱法测定关节克痹丸中大黄素的含量论文.docVIP

　　反相高效液相色谱法测定关节克痹丸中大黄素的含量论文 【关键词】 反相高效液相色谱法；,,关节克痹丸；,,.freelm×150 mm，5 μm），以甲醇0.1%磷酸溶液（70∶30）为流动相，流速1.0 ml/min，检测波长282 nm，柱温40℃。结果大黄素在0.01~0.2 mg/ml范围内线性关系良好（r=0.999 9），平均回收率为97.56%，RSD为1.51%。结论 该法操作简便.freelination of Emodin in Guanjiekebi Pill by RP-HPLC Abstract：ObjectiveAn RP-HPLC method ination of emodin content in Guanjiekebi pill.MethodsZobax C18(4.6 mm×150 mm，5 μm) obile phase consisted of methanol-0.1% phosphoric acid(70∶30).The detection ,the flol/min,and the temperature of the column odin g/ml(r=0.999 9),ethod is simple,sensitive and accurate.It can be applied in quality control of