汉坦病毒S基因0.7kb片段真核载体的构建、表达及基因免疫论文.docVIP

　　汉坦病毒S基因0.7kb片段真核载体的构建、表达及基因免疫论文 .freelent;vaccines.freelation Abstract:AIM To investigate the expression and immuno-genicity of rebinant eukaryotic plasmid containing pe-tent fragment of Hantaan virus S segment.METHODS Re-binant eukaryotic expression vector pcDNA3.1-S0.7ent0.7kb fragment into pcDNA3.1（+）and transfected COS-7cells by lipofectin.Immunoflourescence ice -munoflourescence and ELISA munized mice sera.RESULTS Eukaryotic expression vector pcDNA3.1-S0.7e analysis.Immuno-flourescence and ELISA results shomunized mice.CONCLUSION It is suggested that the0.7kb petent fragment of hant