马蹄金褐色叶斑病菌的生物学特性.pdfVIP

第25卷 第4期 四JII农业大学学报 V()1．25 NO．4 2007年 l2月 Journal of Sichuan Agricultural University Dec．2007 Biological Characteristics of P enophora dichondrae sp．nov． FANG Fang，HUANG Yun”，GAN You—m[n，QIN Yun (())liege of Forestry and Horticulture，Sichuan A cultural University，Yaan 625014，Sichuan，China) Abstract For the growth and ascospore formation of P re,,opkor,,dickondrae sp．nov．，the best medi· um was PDA．the optimum temperature was 25～28℃ and the optimum pH was 5～7．Complete darkness promoted ascospore formation and complete illumination promoted hypha growth．The pathogen could make use of many kinds of carbohydrates，and dextrose seemed to be the best C source， while malt sugar the poorest．Calcium nitrate was the best N source，while ammonium chloride was the poorest．For ascospore germination of the pathogen，the optimum temperature and relative humidity (RH)were 20-28℃and 90％～100％respectively．The optimum pH for ascospore germination was in the range of 6～8．For ascospore germination of the pathogen，the best C source was dextrose and N source was sodium nitrate．The thermal death point of the ascospore was 49℃． Key words：Dichondra repenS；brown leaf blight；Pyrenophora dichondrae sp．nov．；biological char· acteristics 马蹄金褐色叶斑病菌的生物学特性 方 芳，黄 云 ，干友民，秦 芸 (四川农业大学林学园艺学院，四川雅安 625014) 摘要：马蹄金褐色叶斑病菌(Pyrenophora dichondrae sp．nov．)在PDA上生长和产孢最好，菌丝生长和产孢的适温为 25--28℃，适宜pH 5～7，全黑暗促进产孢，全光照促进菌丝生长。对碳源的利用以葡萄糖最好，麦芽糖最差；对氮 源的利用以硝酸钙最好，氯化铵最差。孢子萌发的最适温度为20--28℃，最适相对湿度为90％～100％，最适pH 6 -- 8，最适于孢子萌发的碳源为葡萄糖，氮源是硝酸钠。子囊孢子致死温度是49℃。 关键词：马蹄金；褐色叶斑病；马蹄金核腔菌；生物学特性 中图分类号：S436．8 文献标识码：A 文章编号：1000—2650(2o07)04—0388—04 马蹄金(Dickondra reDe7ls Forst．)俗称金钱草、 病l J等。由马蹄金核腔菌(尸y例0 ora dichon— 黄胆草等，为旋花科马蹄金属多年生的蔓性常绿草 drae sp．nov．)引起的马蹄金褐色叶斑病