骨关节炎患者软骨细胞人类白细胞抗原表达及对外周血单核细胞增殖的影响.pdfVIP

912 主堡耋生匿学~——2007年12月第26卷第12期 Chin J Geriatr，December 2007，Vo1．26，No．12 · 临 床 研 究 · 骨关节炎患者软骨细胞人类白细胞抗原 表达及对外周血单核细胞增殖的影响 王钱 刘爱华 许晓东 黄慈波 【摘要】 目的 通过观察骨关节炎软骨细胞人类白细胞DR抗原(HLA—DR)表达及软骨细胞体 外刺激自体外周血单核细胞(PBMC)增殖反应，探讨软骨细胞的抗原呈递作用。 方法 依据 1986 年美国风湿病学会(ARA)制定的膝骨关节炎诊断标准，收集 16例膝骨关节炎患者软骨标本及5例 股骨颈骨折患者软骨标本，进行软骨细胞培养及白细胞介素一la(IL一1a)刺激后软骨细胞培养，采用流 式细胞技术检测 HLA—DR表达；将软骨细胞与自体PBMC共同培养，采用甲基噻唑基四唑(MTT)法 测定PBMC增殖反应。 结果 对照组和骨关节炎组HLA—DR的表达值( )分别为0．61土0．18和 1．82±0．28；经IL一1a刺激后，两组HLA—DR表达均增高，分别为1．62±0．25和3．89±0．43，骨关节 炎组均高于对照组(P0．01)。对照组和骨关节炎组软骨细胞吸光度(A) 。值分别为0．162±0．018 和0．452±0．035，骨关节炎组明显高于对照组(PO．001)。 结论 膝骨关节炎软骨细胞能够表达 HLA—DR，且经IL一1a刺激后HLA—DR表达增高；骨关节炎软骨细胞有刺激 PBMC增殖的潜能，为其 发挥提呈抗原作用提供更多依据。 【关键词】 骨关节炎，膝； 软骨细胞； HLA—DR抗原； 单核细胞 Chondrocyte HLA—DR expression and its effect on peripheral blood monouclear cells proliferation in osteoarthritis patients W_ANG Qian，LIU Ai—hua，XU Xiao-dong，HU_ANG Ci—bo．Rheumatoid and Immune Department，Beijing Hospital，China Ministry of Health，Beijing j O0 730，China Corresponding author：HUANG Ci．bo，Email：HuangcIbo8966@sina．com [Abstract] 0bjective To approach the antigen presenting role of the chondrocytes through observing the HLA—DR expression on chondrocytes and the peripheral blood monouclear cells proliferation stimulated by autologous chondrocytes． Methods According to the knee osteoarthritis diagnostic criteria instituted by American Rheumatism Association(ARA)in 1 986，articular cartilage tissue of knee ioint from 1 6 osteoarthritis(OA)and articular cartilage tissue of collum femoris from 5 femora1 neck fracture patients were collected．Then these chondrocyte were cultured with or without IL一1 Q．Expression of HLA—DR was assessed by flow cytometry．Peripheral blood mononuclear cells were co—cultured with autologous chondrocytes，and their proliferative response was assessed by methyl thiazolyl tetrazolium (MTT)． Results Th