骨涎蛋白单克隆抗体对亲骨转移乳腺癌细胞与骨基质黏附的抑制.pdfVIP

中国肿瘤生物治疗杂志 · 536· Chinese Journal of Cancer Biotherapy Vo1．14 No．6 Dec．2007 [文章编号] 1007-385X(2007)06-0536-04 ·论 著· 骨涎蛋白单克隆抗体对亲骨转移乳腺癌细胞与骨基质黏附的抑制 龚应静，王 捷 ，宋惠雪，夏 冰(广州军区广州总医院 医学实验科，广州 510010) [摘 要] 目的：探讨骨涎蛋白(bone sialoprotein，BSP)单克隆抗体对亲骨转移乳腺癌细胞(MDA．MB-231．B0)与骨基质黏 附的抑制作用。方法：免疫组化方法检测MDA·MB．231．B0、肺癌细胞SPC．1、大肠癌细胞LOVO、乳腺癌细胞BSP的表达；体 外骨基质黏附实验检测肿瘤细胞与骨基质的黏附，以及BSP单克隆抗体对MDA．MB-231．BO细胞与骨基质黏附的抑制；以 ELISA法检测肿瘤细胞中生长因子TGF．131分泌的变化。结果：免疫组化结果表明，MDA．MB．231．FIO细胞表达BSP，SPCA．1 和LOVO细胞不表达BSP；MDA—MB-231．BO细胞黏附骨基质数明显大于SPCA．1细胞和LOVO细胞(P0．O1)；BSP抗体对 MDA·MB-231·B0细胞和骨基质黏附具有特异抑制作用，随着抗体质量浓度升高(25、50、100、200 g／m1)，其抑制率分别为 22．1％、35．0％、39．2％、41．9％，抑制率与抗体浓度呈正相关；同时BSP抗体能特异性抑制MDA．MB-231．BO细胞与骨基质黏 附所介导TGF·131的释放。结论：BSP单克隆抗体对MDA．MB-231．B0细胞和骨基质黏附具有特异抑制作用，提示BSP抗体 可抑制乳腺癌特异性骨转移。 [关键词] 骨涎蛋白；单克隆抗体；乳腺癌；骨转移；TGF．131 [中图分类号] R737．9；R730．54 [文献标志码] A Inhibitory effects of bone sialoprotein monoclonal antibody on bone-seeking breast cancer cells adhering to bone matrix GONG Ying-jing，WANG Jie ，SONG Hui·xue，XIA Bing(Department of Medical Research，Guangzhou General Hospi- tal，PLA Guangzhou Military Area Command，Guangzhou 510010，China) [Abstract] 0bjective：To determine the inhibito)_v effects of anti—bone sialoprotein(BSP)antibody on human breast cancer cells adhering to bone matrix n vitro．Methods：Expression of BSP in 3 different cancer cell lines(bone seeking breast cancer cell MDA．MB．23 1．BO，lung adenocarcinoma cell SPCA．1 and colon carcinoma cell LOVO)was detected im． mun0hist0chemicanv．Cells adhering test was carried out to investigate the adhering of the 3 different cancer cell lines to mouse bone matrix vitro and the inhibitory effect of anti．BSP antibody on MDA．MB．23 1．BO cells adhering to mouse bone matrix；Enzyme．1inked immunosorbent assay was carried out for quantitative detection of TGF．B．Results：BSP im— munostaining was pos