反相高效液相色谱法测定化妆品中几种防腐剂.pdfVIP

日用化学工业 Vol. 39 No.2 第 39 卷第2 期 April2侃 2酬年4 月 China Surfactant Detergent Cosmetic8 反相高效液相包谱法测定化妆品中几种防腐剂 正健辉1 ，胡家庆2 ，张欣荣1 ，尹笋君1 ，刘小宇3 ，杨峰1 (1.第二阳大学到学院无机化学教研室，川 2刷曰;2. 掏军医学研究所，上梅 2侧33; 3. 第二军医大学墓跚跚生精化学与舟子生辅学教研室，上海 2刷33) 摘蟹:果用反相商效掖相色谱法测定化妆品中重氮烧摹服、对拖基苯叩酸甲醋、对知基苯用酸丙醋3 种防腐剂，用 Water胃 XTerra Ms C 柱(4.6 mm i. d x250 mm ,5 J.Lm) ，乙脯时水作为‘流动相，梯度搅脱，检测搅长254 nmo 回收率为97.30% - I8 102.ω% ，相对标准偏差为3.6% 阳 8.21)岛。 关键词:化妆品;反相商效液相色谱法:防腐剂 中团分SfI;号 :TQ658 X献标惧码:A 文章编号:1ω1 畔 1803(2仪)9)02 -0138 -03 Determination of preservatives in cosmetics by reversed - phase high performance liquid chromatography WAN肌GJ阳i汹阳ioo.缸00翩翩酬唰翩唰 (υ1. De阳m川nent of 加In0耶时盹cCαωhemistr町7仇Ytλt,山he 趾secωon时dMi山监阳阳巧 Medic创叫al Unive阿ersi冒悦sit句y ， 拙Shang}供1且ai 2ω43臼3 ， Chi刷; 2. Naval Institute of Clinical Medicine ,Shanghai 2创)433 ， China; 3. Department of Biochemi脚1 and Molecular Biology ,the Second Military Medical UniveJ曹ity ， Shanghai 2脱)433 ,China) Abstract: A reversed - ph制e high performance liquid chromatography was developed for determination of preservatives in cosmetics ,including diazo alkyl urea ,methyl - p - hydroxy benzoate and propyl - p 四 hy巾Oxy benzoate. A Waters XTerra Ms C18 (5 川，4.6 mm i. d x 250 mm) column w.嗣 used with a mobile ph制e consist of acetonitrile - water and gradient elution. The wavelength 254 nm was selected for detection. 咀le recoveηlies 97. 30% 时 102. ∞% and the relative standard deviation lies 3. 6% - 8. 2% . Key WOI由:cosmetic ;reversed 恤 phase high perforτnance liquid chromato伊aphy ;preservative