响应曲面优化菟丝子多糖提取工艺及抗氧化活性研究.pdfVIP

2015 年第34 卷第8 期 中国酿造 总第 282 期 .35. 研究报告 晌应曲面优化莞丝子多糖提取工艺及抗氧化活性研究 祁小妮1 ，隋 英l ，李振亮1 ，党金宁1 ，刘瑞林2 (1.甘肃民族师范学院化学与生命科学系，甘肃甘南 747000; 2. 西安交通大学医学部药学系，陕西西安 710062) 摘 要:采用响应曲面优化法对莞丝子多糖提取中的料液比、提取温度和提取时间进行优化，确定各因素最佳的水平组合，并对莞丝 子多糖抗氧化活性进行研究。结果表明，在料液比1:25Cg:mL)、提取温度70 c、提取时间90min条件下，蕴丝子多糖提取物中多糖的 含量为7.67%，实际测得莞丝子多糖提取产率与理论预测值相对误差较小。抗氧化活性试验表明，莞丝子多糖能够保护涯基自由基 COHo) 所产生的氧化损伤，对OHo有一定的清除能力，莞丝子多糖的质量浓度为4.0mgl此，最大清除率为18.3%。 关键询:莞丝子:多糖:响应面优化:抗氧化活性 中图分类号:R284.2 文献标识码:A 文章编号:0254-5071 (2015) 08-0035-04 doi:10.1188剖.issn.0254-5071.2015.08.oo7 Optimization ofpolysaccharide extraction technology from C山cu臼 chinensis by response surface methodology and its antioxi也nt acti世ty QI XiaonP , SUI Yingt, LI Zhenliang , DANG Jinning , LIU Ruilin2 臼臼 ρD m 1e.创!J1tofCαhemis自町yandLi曲必 S缸cαlenc臼e~， 鸟 Gans阳uNormaJ 凶l.ln.柏刷Ì1ivl阳V 甲 2之. College 旷o.fpμIha缸rmac叨J只r， 泊X)γ.寸an Jiao Tong Uni附Viersi~饥y， 却XIγP扭 7刀10062， China) j Abs回ct: Using solid-liquid ratio , extraction temperature and time as evaluation factors , the polysaccharide extraction technology 企om Cuscuta chi- nensis was optimized by response surface methodology. The antioxidant activity of C. chmensis was investigated as well. The results showed 也at 也e optimum extraction conditions were extraction temperature 70 C, time 90 min and solid-liquid ratio 1:25 (g:m1). Under these conditions, the theoreti- cal ex田ction efficiency of C. chi11ensis polysaccharides was 7.67% , and there was no significant difference between the actual value and the predict- ed one. The antioxidant e