响应面法优化从玉米皮提取阿魏酸工艺.pdfVIP

姐姐黯回工E眉目 2川 回 晌应面法优化从玉米皮提取阿魏酸工艺 赖红武I ， 2 ，黄 俊I ， 2 ，平丽娟I ， 2 ，毛建卫I ， 2 ，徐昌洪2 (1浙江科技学院生物与化学工程学院，浙江杭州 310023 ,2. 浙江省农产品化学与生物加工技术重点实验室，浙江杭州 310023) 摘 要:采用碱解法从玉米皮中提取阿魏酸，先用 P1 ackett - Burman 法对碱用量、提取时间、提取温度以及碱醇体 和、比等因素进行评价，然后用中心组合实验和响应面法对提取工艺进行优化，采用高效液相色谱法检测阿魏酸含 量。最佳提取条件为 :NaOH 溶液质量分数为1. 034% ，提取温度为 98.4C ，碱醇体积比为 2.556 ，在此条件7 阿 魏酸提取量为1. 798 mg/g 玉米皮。经实验验证后，阿魏酸提取量从1. 522 mg/ g提高到1. 717 mg/g o ;PB 设计;响应面法;优化 关键词:阿魏酸 中圈分类号 :R284.1;R284.2;TS210.9 文献标志码:A 文章编号: 1003-6202 (2013 )02 -0016 -03 Optimization of extraction process of ferulic acid from corn husks using RSA Lai HongwuJ. 2 , Huang Jun1.2 ,Ping Lij uan J. 2 , Mao Jianwei J. 2 , Xu Changhong2 C1. School of Biological and Chemical Engineering , Zhejiang University of Science and Technology , Hangzhou 310023 , China , 2. Zhejiang Provinclal Key Lab for Chem8ιBio Processing Technology of Farm Products ,Hangzhou 310023 ,China) ABSTRACT ,The ferulic acid was extracted from corn husks using alkaline-alcohol hydrolysis method. First , Plackett- Burman CPB) design was used to evaluate the influence of NaOH concentration , extraction temperature and alcohol-alkali volumetric ra tios ,then central composite experiment and response surface analysis were used to optimize the extraction process of ferulic acid from corn husks. The ferulic acid concentration was determined by HPLC. The optimal extraction conditions were as: NaOH contentof 1. 034 %Cw/w) , extraction temperature of 98.4C , alcohol-alkali volumetric ratios of 2. 556. Under the conditions , the ferulic acid concentratio日 was increased from 1. 522 mg/ g to 1. 717 mg/ g. KEYWORDS: ferulic acid; Plackett- Burman design; response surface analysis; optimization 玉米皮是农产品加工中的副产品。玉米皮中含 和化学合成获得。植物中直接摄取方法有碱裂解