响应面试验优化超声辅助提取番茄皮渣中番茄红素工艺及其HPLC-MS测定.pdfVIP

70 2015, Vo1.36, NO.12 画面融事 ※王艺技术 响应面试验优化超声辅助提取番茄皮植中 番茄红素王艺及其HPLC-MS测定 王振强1 ，李孝坤1 ，王浩M (1.黄河水利职业技术学院环境与化学工程系，河南开封 475003; 2.天津科技大学食品工程与生物技术学院， 食品营养与安全省部共建教育部重点实验室，天津 3∞457) 摘 要:以番茄皮渣为原料，采用超声辅助提取工艺，研究提取溶剂种类、超声功率、液固比、提取时间、提取温 度对番茄红素提取效果的影响，通过响应面试验分析，确定最佳工艺条件为:皮渣经95%乙醇溶液处理后，以乙酸 乙酶为提取溶剂，提取温度50.C 、提取时间45min、液固比5:1 (mUg) 、超声功率270W。产物经高效液相色谱­ 质谱测定，番茄红素提取量可达到250.1 mgl峙。通过组织结构观察，与传统提取方法相比，超声提取能有效破坏植 物组织结构，提高提取效果。 关键词z 番茄红素:番茄皮渣:超声辅助提取:优化:测定 Lycopene from Tomato Peel: Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology and Identification by High Performance Liquid Chromatography 1 1 2 WANG Zhenqiang , LI Xiaokun , WANG Ha0 .* (1. Dep缸tment of Environmental and Chemical Engineeri吨， Yellow River Conservancy Technical Institute, Kaifeng 475∞3， China; 2. Key Laboratory of Food Nutrition and Safety, Minis町of Education , School of F，∞d Engineering and Biological Technology , Tianjin University of Science Technology, Tianjin 3∞457， China) Abstract: The extraction of Iycopene from tomato peal by ultrasonic-assisted extraction (UAE) was studied. To obtain the optimal conditions , the effects of operating conditions such as solvent to solid ratio , ultrasonic power, extraction time and temperature on the extraction yield of Iycopene were studied through response surface methodology. The best extraction conditions were determined as follows: pretreatment with 95% ethanol , ethyl acetate as extraction solvent, extraction temperature 50 C , extraction time 45 min , a solvent to solid ratio of 5: 1 (mL/g) and ultrasonic power