北沙参茎叶紫外―可见光谱及薄层色谱鉴别分析.docVIP

北沙参茎叶紫外―可见光谱及薄层色谱鉴别分析 　　摘要：采用紫外-可见光谱法分别对石油醚、乙醚、氯仿、无水乙醇、甲醇和水6种溶剂的北沙参（Glehnia littoralis）茎叶提取液进行紫外-可见光谱扫描，并测定其吸收峰数目和峰位置；以不同配比的石油醚-乙醚-冰醋酸为展开剂，用硅胶G预制薄层板，对上述6种溶剂的北沙参茎叶提取液进行薄层展开，观察其成分的分离情况。结果表明，紫外-可见光谱中，所得的吸收峰数目及峰位置都有差异；薄层色谱主斑点位置有明显差异，且集中、分离度好，均能使其成分得到较好的分离。这两种方法简便、准确、灵敏，可用于北沙参茎叶的快速鉴别 关键词：北沙参（Glehnia littoralis）茎叶；紫外-可见光谱；薄层色谱；分析 中图分类号：S567.5+3；O657.7 文献标识码：A 文章编号：0439-8114（2017）07-1345-03 DOI：10.14088/j.cnki.issn0439-8114.2017.07.037 Ultraviolet-Visible Spectroscopy and Thin-Layer Chromatography Identification of Stems and Leaves of Glehnia littoralis YE Guo-hua， XU Yi-ping （Shandong College of Traditional Chinese Medicine， Yantai 264199， Shandong， China） Abstract：The UV-visible spectra was used to scan the extracts from stems and leaves of Glehnia littoralis with petroleum ether，ethyl ether，chloroform，absolute alcohol，methanol and water，as well as determine the number and position of the absorption peaks. With different ratio of petroleum ether-ethyl ether-glacial acetic acid as developing agent，and using silica gel G to prefabricate thin layer plates，the TLC of the extracts by above 6 kinds of solvent was carried out and the separation of components were observed. The results showed that in UV-visible spectra，there were different number and position of absorption peaks，and the main spot locations had significant differences，with good concentrated and separating degree， both could make a good separation of components of Glehnia littoralis. The two methods were found to be simple，accurate and sensitive，can be used for rapid identification of stems and leaves of Glehnia littoralis. Key words：stems and leaves of Glehnia littoralis； ultraviolet-visible spectrum（UV-visible spectra）； thin layer chromatography 北沙??（Glehnia littoralis）又名莱阳沙参[1]、香草菜根[2]， 性甘味微苦、微寒，归肺、胃经，具有养阴清肺、益胃生津之功效[3]。因传统药用部分是根，而其茎叶部分被废弃。近年来国内外学者对北沙参进行了大量的研究[4]。但对北沙参茎叶的研究报道很少。本试验主要对伞形科植物北沙参茎叶的6种溶剂（石油醚、乙醚、氯仿、乙醇、甲醇、水）提取液进行UV-Vis吸收光谱和TLC色谱扫描并进行研究，为北沙参茎叶的鉴别提供快速、简便的方法。也为北沙参茎叶开发利用、扩大药用和食用资源