海藻渣中岩藻聚糖硫酸酯的提取、纯化及其体外活性分析.pdfVIP

Abstract Fucoidan, an important sulfated polysaccharide in brown seaweed, has many bioactivities, such as anti-virus, anticoagulant, antioxidant and immunomodulation. Laminaria japonica and Lessonia Nigrescen are seaweeds which are commonly used as industrial materials, and their industrial residues contain abundant fucoidan. In this article, industrialresiduesofLaminariajaponica andLessonia Nigrescen wereused asrawmaterial to establish an effective preparation technology of fucoidan. Purification and fraction of fucoidan as well as its chemical composition, antioxidant activity in vitro and PTP1B inhibitionactivitywereanalysed.Theresultsshowedasfollows: 1. Single factor experiment, response surface methodology (RSM) and uniform design were used to optimize the extraction technology of fucoidan fromLaminariajaponica and Lessonia Nigrescen residues by establishing mathematical modeling and regression model. Ultrasound assisted extraction was finally chosen as the optimal extraction method, and comparied with CaCl solution and hot water extraction on the basis of yield and chemical 2 content.The optimal extraction conditionwas as follows:ultrasound time 70min, hotwater extractiontime2h,extractiontemperature90℃,solidtoliquidratio80(mL:g),pH=6. 2.Undertheoptimalcondition,theextractionratesofLFandNFwere4.49%and3.69%, respectively. LF contained higher sulfate content but lower fucose content than that of NF. Themonosaccharide compositionofLFandNF (LPFandNPF) werepurifiedbyHPLC,the molar ratio of fucose and galactose in LPF was 1.03:1, and contained high galactose and fucose.In addition, glucose,mannose, glucuronic acid and a smallamount ofrhamnose and xylose were also detected in LPF.The main monosaccharide ofNPF was fucose, rhamnose and mannose. Galactose, xylose and a small amount of glucose were also found but glucuronicacidwasnotdetectedinNPF.Molecularweight