译文Bioconjugate Technique.doc

Zero-Length Cross-linkers The smallest available reagent systems for bioconjugation are the so-called zerolength 有效 试剂 生物偶联 所谓的 零长度 cross-linkers. These compounds mediate the conjugation of two molecules by 交联剂 化合物 调解 结合 2mol forming a bond containing no additional atoms. Thus, one atom of a molecule is 形成 键 包含 额外的 原子 分子 covalently attached to an atom of a second molecule with no intervening linker or 共价结合 干预链接器 spacer. In many conjugation schemes, the final complex is bound together by virtue of 间隔 方案 合成 绑定在一起 凭借 chemical components that add foreign structures to the substances being cross-linked. 成分 结构体 成分 In some applications, the presence of these intervening linkers may be detrimental to 应用 存在 介入的 连接器 有害的 the intended use. For instance, in the preparation of hapten-carrier conjugates the 预期用途 例如 制备了 半抗体 载体 轭化物 complex is formed with the intention of generating an immune response to the attached hapten. 复杂 形成 生成的意图 免疫反应 附加的 Occasionally, a portion of the antibodies produced by this response 偶尔 一份 抗体 产生 反应 will have specificity for the cross-linking agent used in the conjugation procedure. 特异性 药剂 结合过程 Zero-length cross-linking agents eliminate the potential for this type of cross-reactivity 消除 潜在的 交叉反应 by mediating a direct linkage between two substances. 中介直接联系 化学成分 The reagents described in this chapter can initiate the formation o