乳源性生物活性肽对新生IUGR仔猪肝脏抗氧化功能的影响.pdfVIP

· 12· Animal Husbandry＆Veterinary Medicine 2007 Vo1．39 No．1 1 乳源性生物活性肽对新生IUGR仔猪肝脏 抗氧化功能的影响 杜 伟，王 恬 (南京农业大学动物科技学院，江苏南京 210095) 摘要：本试验选择苏太初生IUGR仔猪lo窝共2o头，配对分为4组：胰岛素组 (I组)、牛乳组 (M组)、酪蛋白酶解产物组 (CH组)和酪蛋白 组 (C组)，饲喂3 d后宰杀。另选5只初生IUGR仔猪立即宰杀，作为0 d对照组 (A组)。M组喂牛乳，I组在牛乳中添加2．5 mg／L胰岛素；C 组用1．5％酪蛋白水溶液取代10％ (、，／V)的牛乳，CH组用1．5％酪蛋白酶解液取代10％ (V／V)的牛乳。宰杀后分析肝脏和血浆中SOD活性和 MDA含量以及血浆中GPT和GOT水平。试验结果显示：酪蛋白酶解物可显著降低肝脏和血浆MDA含量，提高SOD活性 (P0．05)，胰岛素可 显著降低血浆中MDA含量 (P0．05)；3 13龄仔猪GPT和GOT均高于初生仔猪，其中I组、C组和CH组GPT均显著高于M组 (P0．05)。结 果提示：胰岛素和酪蛋白酶解物可提高IUGR仔猪肝脏的抗氧化功能，促进肝细胞的生长发育。 关键词：胰岛素；酪蛋白酶解物；IUGR仔猪；肝脏；抗氧化 中图分类号：S828．5 文献标识码：A 文章编号：0529-5130(2007)l1一oo12一o3 Effects of milk-borne bioactive peptidea on antioxidation functions in the liver of neonatal IUGR pigs DU Wei，WANG Tian (College of Animal Science and Technology，Nanjing Agricultural University，Nanjing 210095，China) Abstract：Twenty neonatal IUGR pigs of one local China pig breed(Sutai)from ten litters were divided into insulin group(I)，milk group (M)，casein group(C)and hydrolysates of casein group(CH)．In addition，five IUGR pigs which were sampled when they were born and arranged as group A．Pigs in groups M and 1 were fed with milk and milk plus 2．5 mg／L insulin，respectively．Pigs in groups CH and C were fed milk substituted 1．5％ hydrolysed casein and 1．5％ casein solution for 10％ (v／v)milk，respectively．Pigs were artificially reared three days and sampled．Maleic dialdehyde(MDA)contents and activities of superoxide dismutase(SOD)in liver and plasma，and activities of GOT and GPT in plasma were determined．The results showed that hydrolysates of casein could reduce MDA contents and enhance the activities of SOD significantly(P0．05)．Insulin could also reduce MDA contents significantly(P0．05)．Howeve