耐多柔比星S180荷瘤小鼠模型的建立.PDFVIP

耐多柔比星S180荷瘤小鼠模型的建立 盛秀胜1 2 ，何忠平 (1.金华职业技术学院，浙江 金华 321017 ；2.金华市食品药品检验所，浙江 金华 321000) 摘要：目的 采用低剂量多柔比星诱导，建立耐多柔比星的 S180 的荷瘤小鼠模型。方法 建立腹水型 S180 荷瘤小鼠模 型。对荷瘤小鼠反复低剂量应用多柔比星，逐步诱导产生耐多柔比星的 S180 荷瘤小鼠模型。并通过多柔比星对 S180 杀 伤率、流式细胞术动态检测 MDR 表达情况等评价模型建立情况。结果 实验组小鼠自用药后 1 周 MDR 表达逐渐增高， 自第5 周后显著性增加(P0.05) ，第 7 周的 P-gp 表达率是第 1 周的近 10 倍。第 5 周时实验组 MDR 阳性表达小鼠占 66.7% ， 第 7 周占 77.8% 。多柔比星对 S180 杀伤率逐月降低。经传代后腹水型小鼠 S180 细胞的表达稳定。结论 本方法简单方 便，可用于在体肿瘤多药耐药研究。 关键词：小鼠模型；耐药；多柔比星；S180 中图分类号：R965.1 文献标志码：A 文章编号：1007-7693(2010)09-0764-04 Establishment of S180 Mouse Model of Multidrug Resistance Induced by Repeatedly Low-Dose Doxorubicin 1 2 SHENG Xiusheng , HE Zhongping (1. Jinhua College of Profession and Technology, Jinhua 321017, China; 2. Jinhua Institute of Food and Drug Control, Jinhua 321000, China) ABSTRACT ：OBJECTIVE To establish the mouse model with multidrug resistance(MDR) induced by repeatedly low-dose doxorubicin. METHODS The mice inoculated by S180 cells were injected with doxorubicin in low dosage. The expression of MDR gene was assayed by flow cytometry and cellular-killed rate was also determined. RESULTS It was found that, in the experiment group, the expression of MDR gene gradually increased after one week of doxorubicin and markedly increased since 5th week of treatment. At 5th week, the percentage of mice with positive MDR gene expression was 66.7% and it was 77.8% at 7th week. And the expression was stable in the cell line. Cellular-killed rate was gradually decreased along with the progress of repeated doxorubicin. CONCLUSION The method is simple and can be used in vivo MDR study. KEY WORDS ：mouse model; resistance; doxorubicin; S180 多药耐药(multidrug resistance，MDR)是肿瘤细 接移植于小鼠，建立小鼠肉瘤模型，同时以低剂 胞免受药物攻击的重要的细胞防御机制，是