复合膳食纤维对严重创伤大鼠肠屏障保护作用的实验性研究.pdfVIP

在损伤早期高于盐水对照组 但各个时间点与盐水对照差异均无显 著性 D 乳酸 伤后各时间点持续维持在高水平 6-12 小时各组 差异无显著意义 24-72 小时膳食纤维和能全力与盐水对照及能全素 组比较差异有显著意义 能全素组在各个时间点与盐水对照差异均 无显著性 结论 1 弥漫性轴索损伤大鼠动物模型复制成功 2 弥漫性轴索 损伤后的大鼠确实存在肠道应激性损伤 3 弥漫性轴索损伤早期给 肠内营养可以降低血中二胺氧化酶和D 乳酸的含量 4 最早期 48 小时之内 单纯的纤维膳食对血中二胺氧化酶和 D 乳酸的降低效 果优于含膳食纤维的复合营养素 5 损伤后期 72 小时及以后 含 膳食纤维的复合肠内营养更有利于血中二胺氧化酶和 D 乳酸的降 低 关键词 弥漫性轴索损伤 肠屏障 二胺氧化酶 乳酸 肠 内营养 复合膳食纤维 3 Experimental Study of Dietary Fiber Complex on Protection of Gut Barrier in Rats after Injury ABSTRACT Objectives: To investigate the protection of various kinds of enteral nutrients on gut barrier function in rats with diffuses brain injury. Methods: Wistar rats with diffuse brain injury, caused by Marmarou’s method, were divided randomly into four groups: they were fed at 3 hours after injury with nature water (group A), enteral nutrition, (Nutrison, group B); enteral nutrition plus dietary fiber complex (Nutrison Fibre, group C); dietary fiber complex (DFC, group D); and controls. The levels of diamine oxidase (DAO) and D-lactate in plasma were measured at 5 time points: 6, 12, 24, 48 and 72 hours after injury. Results:, Marmarou’s models of diffuse brain injury were confirmed under microscope. The concentrations of DAO and D-lactate in plasma increased and the morphological changes were obviously seen in all injury groups at all time points, compared with controls. At 6 to 12 hours after injury, swelling of mucosa was found with infiltration of lymphocytes and neutrophils.