中央内侧丘脑nmda受体在丙泊酚致意识消失中 - 第三军医大学学报.docVIP

中央内侧丘脑NMDA受体丙泊酚致意识消失作用 563000, 贵州省遵义遵义医学院附属医院麻醉科遵义医学院麻醉学系，贵州省麻醉与器官保护基础研究重点实验摘要：目的：探讨中央内侧丘脑中NMDA受体在全身麻醉意识消失中的作用。方法： 48只大鼠微注射模型随机分为4组丙泊酚麻醉后分别于内微量泵注10mM、20mM和40mM的NMDA以及等容量生理盐水。观察微注射后自主体动反应的发生率以及翻正反射恢复时间，并通过组织学对微注射位点进行定位。结果：与C组相比，CMT内微注射NMDA可导致大鼠发生明显的自主体动反应，且翻正反射恢复时间明显缩短（P0.05），增加NMDA浓度可进一步缩短恢复时间（P0.05）；NMDA注射于CMT中时翻正反射恢复时间短于注射部位在CMT外（P0.05）。结论：CMT中微注射NMDA受体激动剂可逆转丙泊酚麻醉，证明了CMT中NMDA受体参与了丙泊酚所导致的意识消失作用。受体: R614.2 NMDA receptors in central medial thalamus participate in propofol-induced unconsciousness. Zhang Yi1,2, Luo Zhuxin2, Wang Yuan2, Duan Zikun2, Li Jia2. （1Department of anesthesiology, Affiliated hospital of Zunyi medical university, 2Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi medical university, Guizhou 563000, China） Abstract: Objective: To investigate the role of NMDA receptors in central medial thalamus in the unconsciousness induced by general anesthesia. Methods: 48 rat models for microinfusion were assigned into 4 groups (n=12). After induction with propofol, 10mM、20mM40mM of NMDA and normal saline with equal volume were microinfused into central medial thalamus. The incidence of purposeful movement and recovery time of righting reflex in each group were observed respectively. Infusion sites were localized by histological method. Results: comparing with group C, infusion of NMDA into CMT elicited notable purposeful movement and reduced the recovery time of righting reflex in rats (P0.05). Increased NMDA dose shorten recovery time further (P0.05). When the microinfusion site localized within CMT, recovery time of righting reflex was remarkably shorter than that out of CMT (P0.05),. Conclusion: Microinfusion of NMDA agonist into CMT reverses propofol anesthesia, indicating that NMDA receptor in CMT may contribute to the propofol-induced unconsciousness. [Key words]: General anesthesia; Central thalamus; NMDA receptor; Loss of consciousness; Intracranial microinjection Supported by the Research Foundation of Education Bureau of Guizhou Pr