基于石墨烯-辣根过氧化物酶修饰电极的过氧化氢传感器.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.12 基于石墨烯-辣根过氧化物酶修饰电极的 过氧化氢传感器 孙颖恩，张秀花，谭允冰，王兰腾，林煦章，朴金花 （华南理工大学轻工与食品学院，广东广州 510641） 摘要：本文采用Hurnmers 方法制备了氧化石墨，通过超声波振荡后得到氧化石墨烯并用水合肼进行还原，制得还原石墨烯，结 果表明，制备的氧化石墨烯材料的层间距由原料石墨的0.33 nm 增加到0.86 nm，结构上出现了含氧基团，还原后的石墨烯某些含氧 基团消失。制备了基于石墨烯修饰辣根过氧化物酶（HRP ）电极的过氧化氢传感器，该传感器以石墨烯（GR）作为酶的载体，壳聚 糖（CS）为粘结剂，硫堇 （Th）作为电子传递材料，玻碳电极（GC）为基体，结果显示，石墨烯和硫堇在修饰电极中有协同作用的 效果，基于 GR-Th-HRP-CS/GC 修饰电极的过氧化氢传感器，具有良好的检测性能，催化电流与 H O 浓度的线性范围为 2 2 -5 -3 -7 K app -5 m 5.0×10 ~1.1×10 mol/L，检测限为9.53×10 mol/L，表观米氏常数 为6.55×10 mol/L，该传感器具有优越的过氧化氢检测性能， 可用于过氧化氢含量检测。 关键词：生物传感器；石墨烯；辣根过氧化物酶；过氧化氢；玻碳电极 文章篇号：1673-9078(2013)12-3046-3052 Hydrogen Peroxide Biosensor Based on Graphene Modified Horseradish Peroxidase Electrode SUN Ying-en, ZHANG Xiu-hua, TAN Yun-bing, WANG Lan-teng, LIN Xu-zhang, PIAO Jin-hua (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: Graphite oxide was prepared by Hummers method. Graphene oxide was obtained from ultrasonic oscillation of graphite oxide and then reduced by using hydrazine hydrate to get graphene (GR). The results showed that the prepared graphene oxide enlarged interlamellar spaces compared to graphite which was from 0.33 nm to about 0.86 nm. The structure of graphene oxide contained many oxygen groups which resulted in the increase of interlamellar sp ace and hydrophilism. After reducion, some oxygen groups disappeared. Hydrogen perox