UPA 重庆医科大学二附院(英文版).pdfVIP

Luo et al. Journal of Experimental Clinical Cancer Research 2011, 30:71 /content/30/1/71 RESEARCH Open Access Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK * Jie Luo, Xin Sun, Feng Gao, Xiaoliang Zhao, Biao Zhong, Hong Wang and Zhijun Sun Abstract Objective: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. Methods: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. Results: 1) Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel. Conclusion: Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin. Introduction cancer, colon cancer, stomach cancer and lung cancer, Breast cancer is one of the major malignant tumors and its mediated-plasminogen activation is dependent threaten women well being. Failure in its treatment on its receptor uPAR in cells. In breast cancer, uPA- mainly arises from cancer proliferation, invasion a