蛋白质工程课堂展示英文PPT.pptVIP

* * Modification?of?KGM?and Study on?the?Application?of?Immobilized Urease Yang Ziyun 01 Background Experimental procedures Conclusion Contents Research meaning Background Konjac Konjac Glucomannan (KGM) What is KGM? Background The?application?of?KGM KGM?is?a?superior?dietary?fiber. It?can be?used to?prevent?and?treat?diseases?such as?Hypertension and Hyperlipidemia. It?has?become?an important?food?additive?and?protective foods?raw material. Urease, is also known as Urea Amidohydrolase. It is a kind of oligomeric enzymes which contains Ni2+. It can specifically catalyze the hydrolysis of urea to release ammonia and carbon dioxide. Background The?definition and application of Urease Research meaning Research significance of the subject Modifying KGM to make it have the properties of immobilized materials. The Urease was selected as the immobilized enzyme. Experimental procedures Experimental methods Oxidation carrier preparation of KGM Steps Determination of free Urease activity、immobilized Urease activity and protein content in samples Extraction of soybean Urease Conclusion Advantages of modified KGM The reaction conditions are mild The temperature range is wider The mechanical strength is better The preservation of the carrier is prolonged after drying The affinity of the substrate is better. Vitality is very common(It can be improved by changing the crosslinking agent).   Thank You * * The picture on the left shows Konjac, its Chinese name is “魔芋”. It is a kind of perennial herb tuber. It?belongs to?the?Araceae?konjac?plant. Konjac is widely distributed in our country.?KGM is the extract of?Konjac?tubers. * * Because urease is a specific enzyme that catalyzes the decomposition of urea, urease has now been used as an analytical reagent for the detection of urea in urine and blood.  * In recent years, researchers used a variety of means（such as the enzymatic hydrolysis）to modify KGM. The film formation and water resistance of KGM were impro