毛细管气相色谱内标法测定核桃油中的脂肪酸.pdfVIP

天然产物研究与开发 Nat Prod Res Dev，2007，19：262-267 文章编号：1001-6880(2007)Suppl-0262-06 毛细管气相色谱内标法测定核桃油中的脂肪酸 张凤枰 ，索有瑞 ，赵先恩 ，王洪伦 ，涂 杰 ，张蓉健 国家粮食储备局成都粮食储藏科学研究所，成都610031； 中国科学院西北 高原生物研究所，西宁810001； l~lJll大学轻纺与食品学院，成都610065 摘 要：测定了核桃油的脂肪酸组成，建立了以十七烷酸甲酯为内标物，用毛细管气相色谱同时测定核桃油五 种脂肪酸(棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸)的方法。样品经氢氧化钾-甲醇皂化、三氟化硼．甲醇酯化后使 生成相应的脂肪酸甲酯，以DM-FFAP毛细管柱分离，火焰离子化检测器测定。用保留时间定性，内标法定量。 结果表明，在所选择的条件下，核桃油脂肪酸在10 rain内能获得较好的分离；五种脂肪酸的峰面积与其质量浓 度有良好的线性关系，相关系数均大于0．998；样品加标回收率(n=6)为92．5％一100．3％，相对标准偏差为 2．41％-4．31％。该方法操作简便、快速、准确，适合批量样品的测定。 关键词：毛细管气相色谱法；内标法；脂肪酸；核桃油 中图分类号：Q946．91；R284．1 文献标识码：A Determination of Fatty Acids in Walnut Oil with Capillary GC by Internal Standard Method ZHANG Feng．ping ，SUO You．rui ’，ZHAO Xian．en ，WANG Hong．1un ，TU Jie ，。，ZHANG Rong-jian Chengdu Grain Storage Research Institute，State Administration ofGrain Reserves，Chengdu 610031，China； Northwest Plateau Institute ofBiology，Chinese Academy ofsc ，Xining 810001，China； College of IndustryTextileFood Engineering ofSichuan University，Cheagdu 610065，China Abstract：The composition of fatty acids in walnut oil was analyzed．A capillary gas chromatographic method for the sim- ultaneous determination ofthe five fatty acids(palmitie acid，ste~e acid，oleic acid，linoleic acid and linolenic acid)in walnut oil was developed and heptadecanoie acid methyl ester was seleeted as the intemal standard substance．The sam． plea were first saponified with KOH-C OH and then the fatty acids were methylated with BF3-CH3 OH，and the obtained fatty acid methyl esters were analyzed by gas chromatography using a DM-FFAP capillary column and a flame ionization detector．Retention time of the peaks could be applied for quantative analysis．Int