二维相关荧光光谱分析技术在食用植物油品质快速检测中的 - 食品科学.pdf

※分析检测 食品科学 2012, Vol. 33, 增刊 1 175 陈 斌，田 萍 * ，赵守敬，陆道礼，邵美丽 (江苏大学食品与生物工程学院，江苏 镇江 212013) 采用荧光发射光谱法并结合二维相关荧光光谱分析技术，对 4 种食用植物油(大豆油、茶树油、菜籽油、 棕榈油) 以及3 个不同浓度梯度的两组分混合食用植物油共 7 种食用植物油样进行快速无损鉴别研究。结果显示：7 种食用植物油样的一维荧光发射光谱谱图在出峰数目、位置以及峰强度上都略有差别，而借助二维荧光光谱对其进 行进一步的研究时，它们的二维谱图在自动峰和交叉峰的出峰数目、位置、峰强度上区别更为清晰直观。表明通 过荧光发射光谱并结合二维相关光谱能够对食用植物油进行有效区分，为鉴别不同植物油种类以及食用油掺杂检测 提供了一种新方法。 二维相关光谱分析；荧光发射光谱；食用植物油 Application of Two-Dimensional Fluorescence Correlation Spectroscopy in Rapid Detection of Edible Vegetable Oil Quality CHEN Bin ，TIAN Ping* ，ZHAO Shou-jing ，LU Dao-li ，SHAO Mei-li (College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China) Abstract：Rapid and non-destructive identification of 4 different types of edible vegetable oils and 3 mixed samples of rapeseed oil and tea tree oil in different proportions was studied using fluorescence emission spectroscopy (FES) combined with two- dimensional fluorescence correlation spectroscopy (2D FCS). The results showed that the one-dimensional fluorescence emis- sion spectra of 7 oil samples slightly varied in the number, location and intensity of peaks. However, clear variations were observed for the two-dimensional fluorescence emission spectra. Therefore, the combined use of FES and 2D FCS permits effective differentiation among edible oil samples as a new method to identify different types of edible vegetable oils and detect adulteration in edible vegetable oils. Key words ：two-dimensional fluorescence correlation spectroscopy (2D FCS)；fluorescence emission spectroscopy (FES)；edible vegetable oil 中图分类号：O657.39 文献标识码：A 文章编号：1002-6630(2012)增刊 1-0175-05 食用植