佛手叶挥发油的成分分析及生物活性研究 - 浙江师范大学_学术期刊社.pdf

373 () Vol.37,No.3 20148 Journal of Zhejiang Normal University(Nat.Sci.) Aug.2014 :1001-5051(2014)03-0329-05 , , , , , (, 321004) :,.,- (GC-MS),,. 40,d-、γ-、、α-、β-,d- γ-50.53%24.05%.、、、 、,(IC )1.1,2.1,6.8,7.2,13.7 50 19.3 mg/mL;Hep3B,HUVEC,Huh7,SP2/0,A549PANC-28. :;;;; :Q949.95 :A Study on constituents and biological activity of volatile oil from fingered citron leaves WEI Yujun, SHAO Linxiang, MA Yanfang, CHENG Wenzhao, GAO Haitao, ZHANG Lumin (College of Chemistry and Life Sciences ,Zhejiang Normal University ,Jinhua Zhejiang 321004,China) Abstract:It was studied the chemical components and biological activity of volatile oil from fingered citron leaves.Volatile oil was extracted from the fingered citron leaves by steam distillation and its chemical compo- nents were analyzed by GC-MS.The effects of volatile oil on bacteria and cancer cells vitality were assayed by nephelometry method and MTT assay,respectively.40 components were identified,and the main components were d-Limonene and γ-Terpinene,accounting for 50.53% and 24.05%,respectively.The results showed that the volatile oil of fingered citron leaves could inhibit the proliferation of Viridans streptococci,Klebsiella pneumonia,Morganella fulton,Escherichia coli,Staphylococcus epidermidis and Hemolytic streptococcus with the IC values of 1.1,2.1,6.8,7.2,13.7 and 19.3mg/mL,respectively.The volatile oil of fingered cit- 50 ron leaves could also inhibit the viability of Hep3B,HUVEC,Huh7,SP2/0,A549 and PANC-28. Key words:fingered citron;volatile oil;constituents;antibacterial activity;anticancer activity (Citrus medica L.var.sarcodactylis (N